DNAPrint: a pathogen-tracking paper sensor platform that integrates functional DNA with nanomaterials

DNAPrint：将功能性 DNA 与纳米材料集成的病原体追踪纸传感器平台

• 批准号: 494302-2016
• 负责人: Li, Yingfu
• 金额: $ 16.32万
• 依托单位: McMaster University
• 依托单位国家: 加拿大
• 项目类别: Strategic Projects - Group
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=657528
• 关键词: DNAPrint; pathogen; tracking; paper; sensor

Many human diseases are linked to infections involving various pathogens. In addition, growing risks of bioterrorism pose on-going threats to public safety and security. Moreover, rapid evolution of antibiotic-resistant "superbugs" is becoming a pressing global concern. It has been recognized that early and accurate pathogen detection represents a vital strategy to prevent large-scale outbreaks. For this reason, engineering simple biosensors for rapid and sensitive pathogen detection represents a strategically important area for fundamental research and technological innovations. We propose to develop "DNAPrint", a novel litmus paper-like sensor device printed with a bio/nanomaterial ink (bioink) to enable rapid and accurate detection of bacterial pathogens. We will focus on the development of DNAPrint technology for Legionella pneumophila, a deadly environmental pathogen. Legionella pneumophila can be found in many natural and man-made water sources such as hot springs, cooling towers, swimming pools, and domestic water systems. It has caused frequent outbreaks in Canada and around the world, and current detection methods are inadequate for field applications - this is because these methods are complicated, need special equipment, and take days to reach conclusive decisions. The proposed DNAPrint technology features printable, portable, litmus paper-like paper sensors that are designed to generate results in as short as a few minutes. This project has the potential to develop an attractive platform technology that places Canada at the forefront of this strategically important area. Towards this goal, we have assembled a multidisciplinary team of three academic groups and three industrial partners that have all the necessary expertise to carry out the proposed work.**********************

许多人类疾病与涉及各种病原体的感染有关。此外，生物恐怖主义的风险日益增加，对公共安全和安保构成持续威胁。此外，具有抗药性的“超级细菌”的快速进化正在成为一个紧迫的全球问题。人们认识到，早期和准确的病原体检测是预防大规模疫情的重要战略。因此，设计简单的生物传感器用于快速和灵敏的病原体检测是基础研究和技术创新的一个重要战略领域。我们建议开发“DNAPrint”，这是一种用生物/纳米材料墨水（bioink）印刷的新型石蕊纸状传感器设备，可以快速准确地检测细菌病原体。我们将专注于开发用于嗜肺军团菌（一种致命的环境病原体）的DNAPrint技术。嗜肺军团菌可以在许多天然和人造水源中找到，如温泉，冷却塔，游泳池和家庭用水系统。它在加拿大和世界各地频繁爆发，目前的检测方法不足以进行现场应用-这是因为这些方法复杂，需要特殊设备，并且需要数天才能得出结论性决定。拟议中的DNAPrint技术具有可打印、便携式、石蕊纸样的纸张传感器，旨在在短短几分钟内生成结果。该项目有可能开发一种有吸引力的平台技术，使加拿大处于这一具有重要战略意义的领域的最前沿。为了实现这一目标，我们组建了一个由三个学术团体和三个工业合作伙伴组成的多学科团队，他们拥有开展拟议工作所需的所有专业知识。