Mechanisms of fatigue pre-conditionning (FPC)

疲劳预调节（FPC）机制

• 批准号: RGPIN-2020-04318
• 负责人: Renaud, JeanMarc
• 金额: $ 2.04万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=739883
• 关键词: Mechanisms; fatigue; pre; conditionning; FPC

Muscle fatigue is defined as a decrease in skeletal muscle force or work when they are repetitively stimulated. The primary mechanism for the decrease in force or work is a decrease in the amount of Ca2+ released by the sarcoplasmic reticulum (SR) resulting in sub-maximal activation of the sarcomere. Lower Ca2+ release reduces the extent of the sarcomere activation, and both events reduce ATP demand by Ca2+ ATPase pumps and myosin ATPase, preserving ATP. This is why fatigue is considered a myoprotective mechanism by which muscles protect themselves from damaging ATP depletion during an activity leading to metabolic stress. One mechanism for the decreased Ca2+ release is an activation of KATP channels, which is activated during any metabolic stress leading to decreased ATP level. KATP channel deficient muscles suffer of severe fiber damage, contractile and metabolic dysfunctions, which further support the notion that fatigue is a myoprotective mechanism. Fatigue pre-conditioning (FPC) is a phenomenon in which a 1st fatigue bout (FAT1) acutely increases the fatigue resistance so that the decreases in tetanic [Ca2+]i and force are much slower during a 2nd fatigue bout (FAT2). Single FDB fibers can be divided into three major groups according to how tetanic [Ca2+]i decreases during FAT1, as FDB muscles is composed of type I, IIA and IIX fibers. Once FPC has been triggered, however, all fibers become fatigue resistant during FAT2. Furthermore, once FPC is triggered, the absence of KATP channel activity no longer causes contractile dysfunctions during FAT2. If muscle fibers no longer rely on KATP channels during FAT2, it can then be suggested that ATP levels are better maintained, especially during the 1st min of fatigue for which ATP levels can decrease by 40-60% during FAT1 OBJECTIVES AND HYPOTHESES. So, the questions are i) what are the mechanisms leading to greater ATP production during FAT2? and ii) what are the intracellular signaling pathways involved in FPC? The long term objective remains the understanding of muscle fatigue mechanisms. AMPK, a cellular energy sensor, modulates glucose uptake, glycogen content and mitochondrial activity in skeletal muscle. Adenosine is produced during metabolic stress. It also modualtes glucose uptake, glycogen content and glycolytic flux in muscle. So, the short term objective is to test the hypothesis that "FPC is triggered by AMPK and adenosine, increasing muscle capacity to better generate ATP during FAT2". Four aims are proposed. Aim 1: show that more ATP is generated during FAT2 than FAT1 via greater glucose uptake, glycogen mobilization and greater mitochondrial pyruvate oxidation. Aim 2: confirm that the level of KATP and ClC-1 Cl-channel activation is much less during FAT2 than FAT1, which will be further evidence that the metabolic stress is much lower during FAT2 than during FAT1. Aim 3: show that AMPK is crucial in triggering FPC. Aim 4: show that adenosine is crucial in triggering FPC.

肌肉疲劳的定义是当骨骼肌受到重复刺激时，其力量或工作的减少。力或功减少的主要机制是肌浆网（SR）释放的Ca2+量减少，导致肌节的次最大激活。较低的Ca2+释放降低了肌节激活的程度，这两种事件都减少了Ca2+ ATP酶泵和肌球蛋白ATP酶对ATP的需求，保留了ATP。这就是为什么疲劳被认为是一种肌肉保护机制，通过这种机制，肌肉在导致代谢压力的活动中保护自己免受ATP损耗的损害。Ca2+释放减少的一种机制是KATP通道的激活，在任何代谢应激导致ATP水平下降时被激活。缺乏KATP通道的肌肉会遭受严重的纤维损伤、收缩和代谢功能障碍，这进一步支持了疲劳是一种肌肉保护机制的观点。疲劳预适应（FPC）是指第一次疲劳（FAT1）急剧增加疲劳阻力，使第二次疲劳（FAT2）时破伤风[Ca2+]i和力的下降速度要慢得多的现象。由于FDB肌肉由i型、IIA型和IIX型纤维组成，根据FAT1过程中强直性[Ca2+]i的减少情况，单个FDB纤维可以分为三大类。然而，一旦FPC被触发，所有纤维在FAT2期间都具有抗疲劳性。此外，一旦FPC被触发，KATP通道活性的缺失不再导致FAT2期间的收缩功能障碍。如果肌肉纤维在FAT2期间不再依赖于KATP通道，那么可以认为ATP水平得到了更好的维持，特别是在疲劳的第1分钟，在FAT1期间ATP水平可以降低40-60%。那么，问题是i)在FAT2过程中导致更多ATP产生的机制是什么？ii) FPC中涉及的细胞内信号通路是什么？长期目标仍然是了解肌肉疲劳机制。AMPK是一种细胞能量传感器，可调节骨骼肌中的葡萄糖摄取、糖原含量和线粒体活性。腺苷在代谢应激过程中产生。它还调节葡萄糖摄取、糖原含量和肌肉中的糖酵解通量。因此，短期目标是验证“FPC是由AMPK和腺苷触发的，在FAT2过程中增加肌肉能力以更好地生成ATP”的假设。提出了四个目标。目的1：表明在FAT2过程中，通过更大的葡萄糖摄取、糖原动员和更大的线粒体丙酮酸氧化，比FAT1产生更多的ATP。目的2：证实FAT2期间的KATP水平和ClC-1 cl -通道激活水平远低于FAT1，这将进一步证明FAT2期间的代谢应激远低于FAT1。目的3：表明AMPK在触发FPC中是至关重要的。目的4：表明腺苷在触发FPC中是至关重要的。