Apparatus for the isolation of specific primary cells from tissue

从组织中分离特定原代细胞的装置

• 批准号: RTI-2022-00416
• 负责人: Scime, Anthony
• 金额: $ 10.92万
• 依托单位: York University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=741836
• 关键词: Apparatus; isolation; specific; primary; cells

NSERC-RTI funds are requested to acquire a platform for conducting fluorescence activated cell sorting (FACS) to isolate primary cells from tissue. Our request is for a state-of-the-art FACS from BD Biosciences (BD FACS MelodyT 2 Laser), which will be a unique addition to be housed in the Farquharson Life Science Building at York University. FACS is the gold standard method to isolate primary cells, based on its precision, flexibility and reliability. It is ideal for the isolation of adult stem cells and fluorescently marked cells from the complex multi-cell population released by tissue digestion. This sophisticated equipment will enable the discrimination between distinct live cell populations based on discrete fluorescence labelling, and then will sort and collect these populations to grow in culture or to be used for further assessments. Importantly, the requested FACS is ideal for use laboratory use: it is user-friendly and does not require a dedicated technician. The use of FACS is integral to the NSERC-funded research programmes of primary applicant Dr. Anthony Scimè and co-applicants Drs. Tara Haas and Emilie Roudier, who are in the School of Kinesiology and Health Science and members of the York University Muscle Health Research Centre (MHRC). The applicants are diverse with respect to gender, ethnicity and career stage, thus providing distinct perspectives and role models to trainees. Each applicant's current and future research hinges on the effective purification of specific primary cell populations from the mixed cell types present within tissues. Currently, there is a dire need for this apparatus, as York University does not possess a 2-laser FACS. At present, FACS must be performed at facilities located several kilometers distant. The lag-time spent transporting the materials (pre and post sort) causes the loss of integrity and excess death of isolated cells. Those facilities also are heavily used and have limited availability. Crucially, these are issues that significantly impede the research progress of the applicants and HQP. The FACS that we intend to purchase will not only be available to meet the immediate and long-term research needs the applicants, but those of several research groups at York University including the Muscle Health research community and others in the Faculties of Health, Science and Engineering. Importantly, many HQP will benefit from the use of this equipment. Trainees will become proficient using the gold standard foundational cell biological approach of FACS to investigate specific cell populations. Having this user-friendly FACS at York will enable more trainees to incorporate this sophisticated technique into their projects, which will elevate the quality and potential impact of their data. It will attract new trainees to join our research programs and will facilitate collaborations of HQP with other researchers.

要求NSERC-RTI资金用于获得进行荧光激活细胞分选（FACS）的平台，以从组织中分离原代细胞。我们要求BD Biosciences提供最先进的流式细胞仪（BD FACS MelodyT 2 Laser），这将是约克大学Farquharson生命科学大楼的一个独特补充。基于其精确性、灵活性和可靠性，FACS是分离原代细胞的金标准方法。它是从组织消化释放的复杂多细胞群中分离成体干细胞和荧光标记细胞的理想方法。这种先进的设备将能够根据离散的荧光标记区分不同的活细胞群体，然后将这些群体分类和收集，以在培养物中生长或用于进一步评估。重要的是，所要求的FACS非常适合实验室用途：它是用户友好的，不需要专门的技术人员。FACS的使用是主要申请人Anthony Scimè博士和共同申请人塔拉哈斯和Emilie Roudier博士的NSERC资助的研究计划的组成部分，他们在运动学和健康科学学院，也是约克大学肌肉健康研究中心（MHRC）的成员。申请人在性别、种族和职业阶段方面各不相同，从而为受训人员提供了独特的视角和榜样。每个申请人当前和未来的研究都取决于从组织内存在的混合细胞类型中有效纯化特定的原代细胞群。目前，由于约克大学没有双激光器FACS，因此迫切需要这种装置。目前，流式细胞仪必须在几公里以外的设施进行。运输材料（分选前和分选后）所花费的滞后时间导致分离细胞的完整性丧失和过度死亡。这些设施也被大量使用，而且可用性有限。至关重要的是，这些问题严重阻碍了申请人和HQP的研究进展。我们打算购买的FACS不仅可以满足申请人的即时和长期研究需求，还可以满足约克大学的几个研究小组的需求，包括肌肉健康研究社区和健康，科学和工程学院的其他研究小组。重要的是，许多HQP将受益于这种设备的使用。学员将熟练使用金标准的基础细胞生物学方法的流式细胞仪来研究特定的细胞群。在约克有了这种方便用户的外地资产管制系统，将使更多的受训人员能够将这种先进的技术纳入其项目，从而提高其数据的质量和潜在影响。它将吸引新的学员加入我们的研究计划，并将促进HQP与其他研究人员的合作。