Engineering isoflavonoid biosynthesis in the forage legume, red clover

草料豆类、红三叶草中异黄酮类生物合成的工程改造

基本信息

  • 批准号:
    RGPIN-2021-02817
  • 负责人:
  • 金额:
    $ 2.4万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Discovery Grants Program - Individual
  • 财政年份:
    2021
  • 资助国家:
    加拿大
  • 起止时间:
    2021-01-01 至 2022-12-31
  • 项目状态:
    已结题

项目摘要

Forage legumes, such as red clover (Trifolium pratense), are an important source of high-quality feed for farm animals. They also provide green manure, improve soil quality, and require low fertilizer input. These features make them a vital component of sustainable farming. Legumes (Fabaceae) characteristically produce a class of specialized metabolites known as isoflavonoids. These compounds can function to attract or deter: signalling for symbiosis with nitrogen-fixing bacteria or warding off harmful microbes, respectively. In red clover, isoflavonoids play an essential role in the defence against fungal infections. The plant is also a source of several isoflavonoids, which provide human health benefits, as nutraceutical supplements. Conversely, the estrogenic capacity of certain isoflavones is of concern in the diet of foraging animals. Therefore, engineering the composition of isoflavonoids in red clover can help produce valuable and resilient varieties. However, the metabolic profile is a complex, multigenic trait that is challenging to engineer. Our long-term goals are to manipulate isoflavonoid biosynthesis in red clover and to confer the ability to produce high-value compounds to synthetic microbes. Recent work suggests that effective regulation of competing flavonoid biosynthesis genes is essential to render the desired metabolic profile. Furthermore, biosynthesis of target isoflavonoids can be improved by identifying auxiliary proteins that form complexes to guide metabolic flux. Establishing an integrated 'omics strategy will help resolve the regulatory, metabolic, structural, and transport components that underlie isoflavonoid composition in red clover. Aim 1: Develop coordinated transcriptomics, proteomics, and targeted metabolomics databases for red clover, with a focus on developmental changes and elicitor-induced responses. Aim 2: Candidate genes, identified using the integrated databases from aim 1, will be functionally characterized to complete unfinished pathways and identify auxiliary proteins. We will also engineer microbes (e.g., Baker's yeast) with plant enzymes to biosynthesize the essential isoflavonoid scaffold. These strains will be used as a plug-and-play tool for gene discovery. Aim 3: Engineered strains will serve as a synthetic biology platform to manufacture high-value isoflavonoids, such as the nutraceutical, coumestrol. In the long-term, our lab will leverage detailed knowledge of isoflavonoid metabolism to identify targets for genome editing and crop enhancement. This application can positively impact agricultural producers of legumes locally in Québec and across Canada. The aims and methodologies of the proposed program will foster excellent HQP training opportunities (2 PhD and 5 BSc students), providing exposure to core and state-of-the-art molecular biology and biochemistry techniques. Our work will translate academic research in specialized metabolism to drive impact in agriculture and human health.
草料豆科植物,如红三叶草(Trifolium pratense),是农场动物优质饲料的重要来源。它们还提供绿肥,改善土壤质量,并且需要较少的肥料投入。这些特点使它们成为可持续农业的重要组成部分。豆类(豆科)的特点是产生一类特殊的代谢物,称为异黄酮。这些化合物可以起到吸引或阻止的作用:分别发出与固氮细菌共生的信号或抵御有害微生物。在红三叶草中,异黄酮在抵抗真菌感染方面起着至关重要的作用。这种植物也是几种异黄酮类化合物的来源,作为营养补充剂对人体健康有益。相反,某些异黄酮的雌激素能力在觅食动物的饮食中是值得关注的。因此,改造红三叶草中异黄酮类化合物的组成可以帮助生产有价值和有弹性的品种。然而,代谢谱是一个复杂的、多基因的特征,对工程来说是具有挑战性的。我们的长期目标是在红三叶草中操纵异黄酮生物合成,并赋予合成微生物生产高价值化合物的能力。最近的研究表明,竞争类黄酮生物合成基因的有效调控是必不可少的,以提供所需的代谢谱。此外,通过鉴定形成复合物的辅助蛋白来指导代谢通量,可以改善目标异黄酮的生物合成。建立一个集成的组学策略将有助于解决调控,代谢,结构和运输成分的基础上的红三叶草异黄酮组成。目标1:为红三叶草开发协调的转录组学、蛋白质组学和靶向代谢组学数据库,重点关注发育变化和激发剂诱导的反应。目标2:使用目标1的集成数据库鉴定的候选基因将被功能表征以完成未完成的途径并鉴定辅助蛋白。我们还将用植物酶设计微生物(例如,贝克酵母)来生物合成必需的异黄酮支架。这些菌株将被用作基因发现的即插即用工具。目标3:工程菌株将作为合成生物学平台来生产高价值的异黄酮类化合物,如营养保健品库美特罗。从长远来看,我们的实验室将利用异黄酮代谢的详细知识来确定基因组编辑和作物增强的目标。这一应用可以对魁省和加拿大各地的豆类农业生产者产生积极影响。该计划的目标和方法将培养优秀的HQP培训机会(2名博士和5名理学士学生),提供核心和最先进的分子生物学和生物化学技术。我们的工作将转化专业代谢的学术研究,以推动农业和人类健康的影响。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Dastmalchi, Mehran其他文献

Purine Permease-Type Benzylisoquinoline Alkaloid Transporters in Opium Poppy
  • DOI:
    10.1104/pp.19.00565
  • 发表时间:
    2019-11-01
  • 期刊:
  • 影响因子:
    7.4
  • 作者:
    Dastmalchi, Mehran;Chang, Limei;Facchini, Peter J.
  • 通讯作者:
    Facchini, Peter J.
Soybean chalcone isomerase: evolution of the fold, and the differential expression and localization of the gene family
  • DOI:
    10.1007/s00425-014-2200-5
  • 发表时间:
    2015-02-01
  • 期刊:
  • 影响因子:
    4.3
  • 作者:
    Dastmalchi, Mehran;Dhaubhadel, Sangeeta
  • 通讯作者:
    Dhaubhadel, Sangeeta
Codeinone reductase isoforms with differential stability, efficiency and product selectivity in opium poppy
  • DOI:
    10.1111/tpj.13975
  • 发表时间:
    2018-08-01
  • 期刊:
  • 影响因子:
    7.2
  • 作者:
    Dastmalchi, Mehran;Chang, Limei;Facchini, Peter J.
  • 通讯作者:
    Facchini, Peter J.
Neopinone isomerase is involved in codeine and morphine biosynthesis in opium poppy
  • DOI:
    10.1038/s41589-019-0247-0
  • 发表时间:
    2019-04-01
  • 期刊:
  • 影响因子:
    14.8
  • 作者:
    Dastmalchi, Mehran;Chen, Xue;Facchini, Peterj
  • 通讯作者:
    Facchini, Peterj
Twin anchors of the soybean isoflavonoid metabolon: evidence for tethering of the complex to the endoplasmic reticulum by IFS and C4H
  • DOI:
    10.1111/tpj.13137
  • 发表时间:
    2016-03-01
  • 期刊:
  • 影响因子:
    7.2
  • 作者:
    Dastmalchi, Mehran;Bernards, Mark A.;Dhaubhadel, Sangeeta
  • 通讯作者:
    Dhaubhadel, Sangeeta

Dastmalchi, Mehran的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Dastmalchi, Mehran', 18)}}的其他基金

Engineering isoflavonoid biosynthesis in the forage legume, red clover
草料豆类、红三叶草中异黄酮类生物合成的工程改造
  • 批准号:
    RGPIN-2021-02817
  • 财政年份:
    2022
  • 资助金额:
    $ 2.4万
  • 项目类别:
    Discovery Grants Program - Individual
Engineering isoflavonoid biosynthesis in the forage legume, red clover
草料豆类、红三叶草中异黄酮类生物合成的工程改造
  • 批准号:
    DGECR-2021-00084
  • 财政年份:
    2021
  • 资助金额:
    $ 2.4万
  • 项目类别:
    Discovery Launch Supplement

相似海外基金

Engineering isoflavonoid biosynthesis in the forage legume, red clover
草料豆类、红三叶草中异黄酮类生物合成的工程改造
  • 批准号:
    RGPIN-2021-02817
  • 财政年份:
    2022
  • 资助金额:
    $ 2.4万
  • 项目类别:
    Discovery Grants Program - Individual
Engineering isoflavonoid biosynthesis in the forage legume, red clover
草料豆类、红三叶草中异黄酮类生物合成的工程改造
  • 批准号:
    DGECR-2021-00084
  • 财政年份:
    2021
  • 资助金额:
    $ 2.4万
  • 项目类别:
    Discovery Launch Supplement
Functional analysis of isoflavonoid biosynthesis related transcription factors using gene co-expression network analysis
利用基因共表达网络分析异黄酮生物合成相关转录因子的功能
  • 批准号:
    23510272
  • 财政年份:
    2011
  • 资助金额:
    $ 2.4万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
BIOSYNTHESIS OF ISOFLAVONOID AND FLAVONOID NUTRIENTS
异黄酮和类黄酮营养素的生物合成
  • 批准号:
    7610258
  • 财政年份:
    2007
  • 资助金额:
    $ 2.4万
  • 项目类别:
SBIR Phase I:Metabolic Engineering of Isoflavonoid Biosynthesis in Eschericia coli
SBIR第一期:大肠杆菌异黄酮生物合成的代谢工程
  • 批准号:
    0712324
  • 财政年份:
    2007
  • 资助金额:
    $ 2.4万
  • 项目类别:
    Standard Grant
BIOSYNTHESIS OF ISOFLAVONOID AND FLAVONOID NUTRIENTS
异黄酮和类黄酮营养素的生物合成
  • 批准号:
    7381642
  • 财政年份:
    2006
  • 资助金额:
    $ 2.4万
  • 项目类别:
BIOSYNTHESIS OF ISOFLAVONOID AND FLAVONOID NUTRIENTS
异黄酮和类黄酮营养素的生物合成
  • 批准号:
    7170879
  • 财政年份:
    2005
  • 资助金额:
    $ 2.4万
  • 项目类别:
Molecular biological analysis of the enzyme networks using the legume isoflavonoid pathway as a model system
使用豆类异黄酮途径作为模型系统对酶网络进行分子生物学分析
  • 批准号:
    15510183
  • 财政年份:
    2003
  • 资助金额:
    $ 2.4万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Biosynthesis of Isoflavonoid Phytoalexins in Pea
豌豆中异黄酮植物抗毒素的生物合成
  • 批准号:
    9319733
  • 财政年份:
    1994
  • 资助金额:
    $ 2.4万
  • 项目类别:
    Standard Grant
Biosynthesis of Isoflavonoid Phytoalexins in Pea
豌豆中异黄酮植物抗毒素的生物合成
  • 批准号:
    8916265
  • 财政年份:
    1990
  • 资助金额:
    $ 2.4万
  • 项目类别:
    Continuing Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了