Functional analysis of isoflavonoid biosynthesis related transcription factors using gene co-expression network analysis

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In order to isolate transcription factors involved in isoflavonoid biosynthesis, we established an EST (expressed sequence tag) data in Iris germanica root cultures, accumulating isoflavonoid, using next-generation sequencing instrument illumina HiSeq 1000 with 100 bp paired-end reads. Furthermore, we carrying out transcript expression analysis (using a custom Iris DNA array) in subculturing time-course experiment of Iris germanica root cultured cells for gene discovery in gene co-expression network analysis.We established standalone Confeito Java-GUI (gene co-expression network analysis) software that extracted highly-interconnected gene modules. Using the public Arabidopsis DNA array resource data (more than 9 thousands), we have shown an application of our developed Confeito Java-GUI software to isolate gene co-expression network module in Arabidopsis aliphatic glucosinolate biosynthesis.