Imaging protein crystals for identification of submicron nucleation and growth optimization
对蛋白质晶体进行成像以识别亚微米成核和生长优化
基本信息
- 批准号:RTI-2022-00252
- 负责人:
- 金额:$ 8.95万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Research Tools and Instruments
- 财政年份:2021
- 资助国家:加拿大
- 起止时间:2021-01-01 至 2022-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The study of the three-dimensional structure of proteins provides invaluable insight into the fundamental mechanisms of biological processes. X-ray crystallography provides a fantastic means to solve protein structures at atomic resolution. The three co-applicants in this proposal have an urgent need for such knowledge to pursue exciting Discovery Programs in the structural biology of membrane proteins (Martin Audet), enzymology of caspases (Jean-Bernard Denault), and G-protein cell biology (Christine Lavoie). As detailed in the proposal, the co-applicants would greatly benefit from a high-resolution description of proteins and cofactors involved in regulating the molecular systems they studied. Martin Audet is an early career researcher with a research program that focuses on X-ray crystallography-based structural biology of solute carrier transporters. Jean-Bernard Denault studies mechanisms of caspase activation, regulation, and substrate recognition. Finally, Christine Lavoie focuses on the nonconventional cellular roles of the heterotrimeric GalphaS protein, specifically the role of ubiquitination on the structure function of GalphaS. To support their NSERC research programs and HQP training, the co-applicants require access to equipment that would allow them to image and screen a large number of crystallization conditions to determine high-resolution structures of important proteins in their systems using an X-ray crystallography approach. Imager robots for the identification of protein crystals use multiple detection strategies to address crystallization screens of high complexity (dozens of thousands of conditions) and allow easy retrieval and classification of protein crystals in a timely and reproducible manner. The current lack of such equipment in Sherbrooke severely impedes advances in these Discovery Programs. Automatization of the imaging of the crystallization plates and the availability of detection methods adapted for protein crystals by the requested equipment will unlock the research program of the co-applicants, allow us to start new collaborations with other academic and industrial partners, and finally provide HQPs with an instrument that will elevate their skill set with state-of-the-art imaging technology in X-ray protein crystallography. This expertise is rare in Canada and HQPs that are proficient in this field is in high demand in the industry. The requested instrument will enable a solid ground to train HQPs that will constitute a future workforce to propagate the leverage of structural biology and X-ray crystallography in the Canadian scientific industry and academia.
蛋白质三维结构的研究为了解生物过程的基本机制提供了宝贵的见解。X射线晶体学提供了一种以原子分辨率解决蛋白质结构的奇妙方法。本提案中的三位共同申请人迫切需要这些知识,以在膜蛋白的结构生物学(Martin Audet)、半胱天冬酶的酶学(Jean-Bernard Denault)和G蛋白细胞生物学(克莉丝汀Lavoie)方面开展令人兴奋的发现计划。正如提案中所详述的那样,共同申请人将极大地受益于对参与调节他们所研究的分子系统的蛋白质和辅因子的高分辨率描述。Martin Audet是一名早期职业研究员,其研究项目专注于溶质载体转运蛋白的基于X射线晶体学的结构生物学。Jean-Bernard Denault研究半胱天冬酶激活、调节和底物识别的机制。最后,克莉丝汀Lavoie专注于非传统的细胞角色的异源三聚体的GalphaS蛋白,特别是泛素化的作用上的结构功能的GalphaS。为了支持他们的NSERC研究计划和HQP培训,共同申请人需要获得设备,使他们能够成像和筛选大量结晶条件,以使用X射线晶体学方法确定其系统中重要蛋白质的高分辨率结构。用于识别蛋白质晶体的成像机器人使用多种检测策略来解决高复杂性(成千上万种条件)的结晶筛选,并以及时和可重复的方式轻松检索和分类蛋白质晶体。目前舍布鲁克缺乏这种设备,严重阻碍了这些发现计划的进展。结晶板成像的自动化以及所需设备适用于蛋白质晶体的检测方法的可用性将解锁共同申请人的研究计划,使我们能够与其他学术和工业合作伙伴开始新的合作,并最终为HQP提供一种仪器,该仪器将提高他们在X射线蛋白质晶体学中最先进的成像技术的技能。这种专业知识在加拿大很少见,业内对精通该领域的HQP的需求很高。所要求的仪器将为培训HQP奠定坚实的基础,这些HQP将构成未来的劳动力,以在加拿大科学工业和学术界传播结构生物学和X射线晶体学的杠杆作用。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Audet, Martin其他文献
Protein-protein interactions monitored in cells from transgenic mice using bioluminescence resonance energy transfer
- DOI:
10.1096/fj.09-144816 - 发表时间:
2010-08-01 - 期刊:
- 影响因子:4.8
- 作者:
Audet, Martin;Lagace, Monique;Bouvier, Michel - 通讯作者:
Bouvier, Michel
Small-scale approach for precrystallization screening in GPCR X-ray crystallography
- DOI:
10.1038/s41596-019-0259-y - 发表时间:
2020-01-01 - 期刊:
- 影响因子:14.8
- 作者:
Audet, Martin;Villers, Kelly;Stevens, Raymond C. - 通讯作者:
Stevens, Raymond C.
Subcellular imaging of dynamic protein interactions by bioluminescence resonance energy transfer
- DOI:
10.1529/biophysj.107.117275 - 发表时间:
2008-02-01 - 期刊:
- 影响因子:3.4
- 作者:
Coulon, Vincent;Audet, Martin;Perroy, Julie - 通讯作者:
Perroy, Julie
Insights into signaling from the β2-adrenergic receptor structure
- DOI:
10.1038/nchembio.97 - 发表时间:
2008-07-01 - 期刊:
- 影响因子:14.8
- 作者:
Audet, Martin;Bouvier, Michel - 通讯作者:
Bouvier, Michel
Audet, Martin的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Audet, Martin', 18)}}的其他基金
A multiscale structural understanding of organic anion transporting polypeptide transporters (OATPs) functions
对有机阴离子转运多肽转运蛋白 (OATP) 功能的多尺度结构理解
- 批准号:
RGPIN-2021-03486 - 财政年份:2022
- 资助金额:
$ 8.95万 - 项目类别:
Discovery Grants Program - Individual
A multiscale structural understanding of organic anion transporting polypeptide transporters (OATPs) functions
对有机阴离子转运多肽转运蛋白 (OATP) 功能的多尺度结构理解
- 批准号:
DGECR-2021-00185 - 财政年份:2021
- 资助金额:
$ 8.95万 - 项目类别:
Discovery Launch Supplement
A multiscale structural understanding of organic anion transporting polypeptide transporters (OATPs) functions
对有机阴离子转运多肽转运蛋白 (OATP) 功能的多尺度结构理解
- 批准号:
RGPIN-2021-03486 - 财政年份:2021
- 资助金额:
$ 8.95万 - 项目类别:
Discovery Grants Program - Individual
相似国自然基金
子宫内膜间质与巨噬细胞之间通过Protein S-MerTK-Apelin信号对
话促进子宫腺肌病蜕膜化缺陷的机制研究
- 批准号:
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
有翅与无翅蚜虫差异分泌唾液蛋白Cuticular protein在调控植物细胞壁免疫中的功能
- 批准号:32372636
- 批准年份:2023
- 资助金额:50.00 万元
- 项目类别:面上项目
原发性开角型青光眼中SIPA1L1促进小梁网细胞外基质蛋白累积升高眼压的作用机制
- 批准号:82371054
- 批准年份:2023
- 资助金额:49.00 万元
- 项目类别:面上项目
胆固醇合成蛋白CYP51介导线粒体通透性转换诱发Th17/Treg细胞稳态失衡在舍格伦综合征中的作用机制研究
- 批准号:82370976
- 批准年份:2023
- 资助金额:48.00 万元
- 项目类别:面上项目
细胞周期蛋白依赖性激酶Cdk1介导卵母细胞第一极体重吸收致三倍体发生的调控机制研究
- 批准号:82371660
- 批准年份:2023
- 资助金额:49.00 万元
- 项目类别:面上项目
转运蛋白RCP调控巨噬细胞脂肪酸氧化参与系统性红斑狼疮发病的机制研究
- 批准号:82371798
- 批准年份:2023
- 资助金额:49.00 万元
- 项目类别:面上项目
G蛋白偶联受体GPR110调控Lp-PLA2抑制非酒精性脂肪性肝炎的作用及机制研究
- 批准号:82370865
- 批准年份:2023
- 资助金额:49.00 万元
- 项目类别:面上项目
新型小分子蛋白—人肝细胞生长因子三环域(hHGFK1)抑制破骨细胞及治疗小鼠骨质疏松的疗效评估与机制研究
- 批准号:82370885
- 批准年份:2023
- 资助金额:49.00 万元
- 项目类别:面上项目
蛋白精氨酸甲基化转移酶PRMT5调控PPARG促进巨噬细胞M2极化及其在肿瘤中作用的机制研究
- 批准号:82371738
- 批准年份:2023
- 资助金额:49.00 万元
- 项目类别:面上项目
紧密连接蛋白PARD3下调介导黏膜上皮屏障破坏激活STAT3/SNAI2通路促进口腔白斑病形成及进展的机制研究
- 批准号:82370954
- 批准年份:2023
- 资助金额:47.00 万元
- 项目类别:面上项目
相似海外基金
Elucidation of plasticity and brittleness of protein crystals based on dislocation theory
基于位错理论阐明蛋白质晶体的塑性和脆性
- 批准号:
23H01305 - 财政年份:2023
- 资助金额:
$ 8.95万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Japan_IPAP: Embedding protein crystals within synthetic tissues as catalytic soft materials
Japan_IPAP:将蛋白质晶体嵌入合成组织中作为催化软材料
- 批准号:
BB/X01259X/1 - 财政年份:2023
- 资助金额:
$ 8.95万 - 项目类别:
Research Grant
RNA delivery by self-assembling phagocytic-competent protein crystals
通过自组装具有吞噬能力的蛋白质晶体进行 RNA 递送
- 批准号:
10067767 - 财政年份:2023
- 资助金额:
$ 8.95万 - 项目类别:
Collaborative R&D
Designing Animal-Free Organoids Based on Engineered Vegetables and Protein Crystals [VegFold]
基于工程蔬菜和蛋白质晶体设计无动物源类器官 [VegFold]
- 批准号:
2748712 - 财政年份:2022
- 资助金额:
$ 8.95万 - 项目类别:
Studentship
Development of an automatic integration range determination method for pulsed neutron diffraction data collected from protein single crystals
开发从蛋白质单晶收集的脉冲中子衍射数据的自动积分范围确定方法
- 批准号:
22K06157 - 财政年份:2022
- 资助金额:
$ 8.95万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Designing animal-free organoids based on engineered vegetables and protein crystals [VegFold]
基于工程蔬菜和蛋白质晶体设计非动物类器官 [VegFold]
- 批准号:
2765722 - 财政年份:2022
- 资助金额:
$ 8.95万 - 项目类别:
Studentship
Nucleation and Growth of Membrane Protein Crystals
膜蛋白晶体的成核和生长
- 批准号:
2624982 - 财政年份:2021
- 资助金额:
$ 8.95万 - 项目类别:
Studentship
Cryopreservation of antifreeze protein multicellular systems based on X-ray nanoscale observations in vivo for ice crystals
基于体内冰晶 X 射线纳米级观察的抗冻蛋白多细胞系统的冷冻保存
- 批准号:
21K14466 - 财政年份:2021
- 资助金额:
$ 8.95万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Three dimensional nanostructure fabrication by using protein crystals as the templete
以蛋白质晶体为模板构建三维纳米结构
- 批准号:
20K05269 - 财政年份:2020
- 资助金额:
$ 8.95万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
High throughput Imaging System for analysis of protein crystals
用于蛋白质晶体分析的高通量成像系统
- 批准号:
439226007 - 财政年份:2020
- 资助金额:
$ 8.95万 - 项目类别:
Major Research Instrumentation