Oxygen-Regulated Tissue Culturing for Accurate In Virto Cell Analyses

氧调节组织培养可进行准确的体外细胞分析

基本信息

  • 批准号:
    RTI-2022-00489
  • 负责人:
  • 金额:
    $ 10.93万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Research Tools and Instruments
  • 财政年份:
    2021
  • 资助国家:
    加拿大
  • 起止时间:
    2021-01-01 至 2022-12-31
  • 项目状态:
    已结题

项目摘要

We request a gas-controlled cell culturing station that allows the establishment and culturing of cells under conditions of oxygen that are similar to what cells encounter in the body (normoxia). Culturing of cells under ambient (20%) oxygen concentrations that is usually done causes many cells to rapidly stop growing due to the oxidative stress that impacts many growth and repair pathways. In contrast, culturing cells in 2-5% oxygen has been shown to significantly extend the replicative lifespan of mouse cells from 5-10 cell divisions to >60, to increase their growth rate and to alter DNA repair pathways, showing that oxidative stress strongly impacts cells once they are removed from the body. Members of our group rely heavily upon cell culturing in vitro for our basic research programs. Although our group has two small oxygen-regulated incubators that partly address this problem of chronic oxidative stress, we do not have the tissue culturing facilities needed for proper establishment and culturing of cells that are needed to accurately examine the induction and repair of DNA damage, the regulation of cell replicative lifespan in different species and the factors involved in regulating the formation and activity of membraneless organelles. Without the ability to rigorously control oxygen levels in the absence of the oxygen spikes that are encountered in standard culturing hoods, the results of in vitro experiments do not represent conditions in vivo. This tissue culturing facility will address this shortcoming, be the only one at our university and would provide the members of our group, and the 48 trainees and personnel in our seven labs a unique ability to advance their training using a state-of-the-art culturing facility. Otherwise unattainable data in areas of cell aging, DNA repair and membraneless organelle formation and function will promote the careers of our developing pool of HQP and promote expansion of our training program in our institution and in other labs in Canada that we collaborate with.
我们要求一种气体控制的细胞培养站,其允许在与细胞在体内遇到的氧气(常氧)类似的氧气条件下建立和培养细胞。通常在环境(20%)氧浓度下培养细胞会导致许多细胞由于氧化应激而迅速停止生长,氧化应激会影响许多生长和修复途径。相比之下,在2-5%氧气中培养细胞已显示出将小鼠细胞的复制寿命从5-10次细胞分裂显著延长至>60次,以增加它们的生长速率并改变DNA修复途径,这表明一旦将细胞从体内移除,氧化应激就会强烈影响细胞。我们小组的成员在很大程度上依赖于我们的基础研究计划体外细胞培养。虽然我们的团队有两个小型氧气调节培养箱,部分解决了慢性氧化应激的问题,但我们没有适当建立和培养细胞所需的组织培养设施,这些细胞需要准确检查DNA损伤的诱导和修复,不同物种中细胞复制寿命的调节以及参与调节无膜细胞器形成和活性的因素。在没有标准培养罩中遇到的氧气峰值的情况下,没有严格控制氧气水平的能力,体外实验的结果不能代表体内条件。这个组织培养设施将解决这个缺点,是我们大学唯一的一个,将为我们小组的成员,以及我们七个实验室的48名学员和人员提供一种独特的能力,使用最先进的培养设施来推进他们的培训。在细胞老化、DNA修复和无膜细胞器形成和功能领域无法获得的数据将促进我们发展中的HQP人才库的职业生涯,并促进我们在我们机构和加拿大其他实验室合作的培训计划的扩展。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Riabowol, Karl其他文献

ING3 protein expression profiling in normal human tissues suggest its role in cellular growth and self-renewal
  • DOI:
    10.1016/j.ejcb.2015.03.002
  • 发表时间:
    2015-05-01
  • 期刊:
  • 影响因子:
    6.6
  • 作者:
    Nabbi, Arash;Almami, Amal;Riabowol, Karl
  • 通讯作者:
    Riabowol, Karl
Isolation of Pure Nuclei Using a Sucrose Method.
  • DOI:
    10.1101/pdb.prot083741
  • 发表时间:
    2015-08-03
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Nabbi, Arash;Riabowol, Karl
  • 通讯作者:
    Riabowol, Karl
ING function in apoptosis in diverse model systems
  • DOI:
    10.1139/o08-107
  • 发表时间:
    2009-02-01
  • 期刊:
  • 影响因子:
    2.9
  • 作者:
    Shah, Sitar;Smith, Heather;Riabowol, Karl
  • 通讯作者:
    Riabowol, Karl
HSP70 induction by ING proteins sensitizes cells to tumor necrosis factor alpha receptor-mediated apoptosis
  • DOI:
    10.1128/mcb.01538-06
  • 发表时间:
    2006-12-01
  • 期刊:
  • 影响因子:
    5.3
  • 作者:
    Feng, Xiaolan;Bonni, Shirin;Riabowol, Karl
  • 通讯作者:
    Riabowol, Karl
ING1a expression increases during replicative senescence and induces a senescent phenotype
  • DOI:
    10.1111/j.1474-9726.2008.00427.x
  • 发表时间:
    2008-12-01
  • 期刊:
  • 影响因子:
    7.8
  • 作者:
    Soliman, Mohamed A.;Berardi, Philip;Riabowol, Karl
  • 通讯作者:
    Riabowol, Karl

Riabowol, Karl的其他文献

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{{ truncateString('Riabowol, Karl', 18)}}的其他基金

Program in mammalian telomere biology
哺乳动物端粒生物学项目
  • 批准号:
    RGPIN-2017-04871
  • 财政年份:
    2021
  • 资助金额:
    $ 10.93万
  • 项目类别:
    Discovery Grants Program - Individual
Program in mammalian telomere biology
哺乳动物端粒生物学项目
  • 批准号:
    RGPIN-2017-04871
  • 财政年份:
    2020
  • 资助金额:
    $ 10.93万
  • 项目类别:
    Discovery Grants Program - Individual
Program in mammalian telomere biology
哺乳动物端粒生物学项目
  • 批准号:
    RGPIN-2017-04871
  • 财政年份:
    2019
  • 资助金额:
    $ 10.93万
  • 项目类别:
    Discovery Grants Program - Individual
Program in mammalian telomere biology
哺乳动物端粒生物学项目
  • 批准号:
    RGPIN-2017-04871
  • 财政年份:
    2018
  • 资助金额:
    $ 10.93万
  • 项目类别:
    Discovery Grants Program - Individual
Program in mammalian telomere biology
哺乳动物端粒生物学项目
  • 批准号:
    RGPIN-2017-04871
  • 财政年份:
    2017
  • 资助金额:
    $ 10.93万
  • 项目类别:
    Discovery Grants Program - Individual

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