Speciation and extinction risk in the vascular plants of Canada

加拿大维管植物的物种形成和灭绝风险

• 批准号: RGPIN-2019-04141
• 负责人: Vamosi, Jana
• 金额: $ 3.42万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=744846
• 关键词: Speciation; extinction; risk; vascular; plants

Of the 5211 known species of vascular plants in Canada, there are >650 at some level of risk of extinction. Determining the level of risk for each species is a time-consuming endeavor, leading scientists to attempt to devise predictors of extinction risk to produce short-lists of candidate species deserving the most immediate attention. We are just beginning to understand how the genome is altered with speciation and how it affects the phenotype of an organism. While animal species can rarely tolerate an extra chromosome in their genomes (e.g., most human "trisomies" result in miscarriage or developmental disorders), plants readily incorporate an entire extra chromosomal set. This process can result in speciation because the individuals with extra chromosome sets (polyploids) often cannot produce viable offspring with their diploid (two sets of chromosomes) relatives. Whether polyploid species exhibit differences in their ability to adapt to changing environments is not understood. This proposal aims to uncover the consequences of polyploid speciation to the extinction risk of plant species within Canada with three short-term objectives. In the first objective, I will produce better estimates of the rate of polyploid speciation in important Canadian lineages. There are approximately 42 vascular plant species endemic to Canada, and many are members of familiar elements of the Canadian flora, such as the willows, hawthorns, and goldenrods. Despite established methodology for determining genome size, these genera have >50% of their species with unknown ploidy. My lab will use flow cytometry, species distribution modelling, and phylogenetics to identify where polyploidization has occurred in the evolutionary history of these lineages and then test hypotheses of how these processes have affected their current extinction risk. In the second objective, we will survey the change in floral phenology amongst flowering plants with different ploidy levels using herbarium specimens to document trait change over the past century. Finally, in the third objective, I will examine how pollination is altered upon shifts in ploidy. Polyploids are thought to be more often self-compatible and less dependent on pollinators and these differences may be critical in predicting how populations will respond to ecological changes such as climatic warming or habitat modification. Currently, we lack information on whether species-at-risk in Canada are disproportionately polyploid, or of recent origin. Answers to these questions will contribute to our understanding of what drives differences in speciation and extinction rate, but will also lead to effective policy for prioritizing species for conservation effort. This proposal will examine Canada's endemic vascular plant species in terms of their evolutionary history to uncover the role of speciation history, polyploidization, and specialization in making species vulnerable to human disturbance.

在加拿大已知的5211种维管植物中，有大约650种处于某种程度的灭绝危险。确定每个物种的风险水平是一项耗时的努力，这使得科学家们试图设计出灭绝风险的预测器，以列出最值得立即关注的候选物种的短名单。我们刚刚开始了解基因组是如何随着物种形成而改变的，以及它是如何影响生物体的表型的。虽然动物物种很少能在它们的基因组中容忍一条额外的染色体（例如，大多数人类“三体”导致流产或发育障碍），但植物很容易包含一整套额外的染色体。这一过程可能导致物种形成，因为具有额外染色体组（多倍体）的个体通常不能与其二倍体（两组染色体）亲属产生有活力的后代。多倍体物种在适应环境变化的能力上是否表现出差异尚不清楚。该提案旨在揭示多倍体物种形成对加拿大植物物种灭绝风险的影响，并有三个短期目标。在第一个目标中，我将对加拿大重要谱系中多倍体物种形成率做出更好的估计。大约有42种维管植物是加拿大特有的，其中许多都是加拿大植物群中熟悉的元素，如柳树、山楂和黄花。尽管已经建立了确定基因组大小的方法，但这些属有50%的物种具有未知的倍性。我的实验室将使用流式细胞术、物种分布模型和系统遗传学来确定这些谱系的进化史中多倍体化发生的位置，然后测试这些过程如何影响它们当前灭绝风险的假设。在第二个目标中，我们将利用植物标本馆标本来记录过去一个世纪中不同倍性水平开花植物的花物候变化。最后，在第三个目标中，我将研究传粉是如何随着倍性的变化而改变的。多倍体通常被认为是自交的，对传粉者的依赖较少，这些差异可能对预测种群如何应对气候变暖或栖息地改变等生态变化至关重要。目前，我们缺乏关于加拿大面临风险的物种是不成比例的多倍体，还是最近起源的信息。这些问题的答案将有助于我们理解是什么导致了物种形成和灭绝率的差异，但也将导致有效的政策，优先考虑物种的保护工作。本研究将研究加拿大特有的维管植物物种的进化史，以揭示物种形成史、多倍体化和特化在物种易受人类干扰中的作用。