Airborne environmental DNA as a new applied tool for industry, research and regulatory approaches

空气环境 DNA 作为工业、研究和监管方法的新应用工具

• 批准号: 580884-2022
• 负责人: Clare, ElizabethEL
• 金额: $ 2.15万
• 依托单位: York University
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=745181
• 关键词: Airborne; environmental; DNA; new; applied

Monitoring biodiversity is a commercially important business estimated at $10 billion annually. Demand includes government regulatory monitoring and sectors such as oil and gas, natural resource management, financial and supply chain interests and activities such as assessing the success of bio-remediation. Because measurement of biodiversity is challenging researchers and companies are increasingly incorporating DNA-based tools to refine these measurements.A DNA sample is normally collected directly from an individual, as a blood sample, piece of tissue or perhaps a cheek swab. However, all organisms also leave environmental (e)DNA behind like footprints. Collecting this material has become particularly useful in aquatic systems as a non-invasive method of biomonitoring. The Clare Lab recently developed a prototype sampling device and demonstrated that eDNA can be filtered directly out of air samples. This is a tremendous new tool to monitor terrestrial biodiversity. NatureMetrics is the world's largest eDNA analytical company with headquarters in the UK and NatureMetrics North America located in Guelph Ontario. In this proposal, NatureMetrics North America and the Clare Lab are teaming up to develop air-based sampling as a new commercial offering. They will use the prototype developed by the Clare Lab and a specialised field location to validate a sampling strategy to accurately assess animal communities e.g., in roosts and caves. Validation will include the timing and deployment of sampling devices and modeling species recovery. Success will allow NatureMetrics North American to become the first company in the world providing eDNA air sampling as a commercial service.

监测生物多样性是一项重要的商业活动，估计每年价值100亿美元。需求包括政府监管监测以及石油和天然气、自然资源管理、金融和供应链利益等部门，以及评估生物补救成功与否等活动。由于生物多样性的测量具有挑战性，研究人员和公司越来越多地采用基于DNA的工具来完善这些测量。DNA样本通常直接从个体收集，如血液样本，组织块或脸颊拭子。然而，所有生物也会像足迹一样留下环境（e）DNA。收集这种材料已成为特别有用的水生系统作为一种非侵入性的生物监测方法。克莱尔实验室最近开发了一种原型采样装置，并证明eDNA可以直接从空气样本中过滤出来。这是监测陆地生物多样性的一个巨大的新工具。Naturedom是全球最大的eDNA分析公司，总部位于英国，Naturedom北美总部位于安大略的圭尔夫。在这项提案中，Naturemont North America和克莱尔Lab将合作开发基于空气的采样，作为一种新的商业产品。他们将使用克莱尔实验室开发的原型和专门的现场位置来验证采样策略，以准确评估动物群落，栖息在洞穴里验证将包括采样装置的时间安排和部署以及物种恢复建模。这一成功将使北美自然科学公司成为世界上第一家提供eDNA空气采样商业服务的公司。