Shaking incubators and accessories for the study of La function and PHD proteins in yeast

该申请由约克大学的两位主要研究人员提交，申请资金用于购买两台摇床孵化器和附件，用于专门生长和研究酵母裂糖酵母和酿酒酵母。对于主要申请人，该设备将专门用于S. pombe的生长，以建立遗传筛选，寻找在细胞生长，代谢和细胞对环境应激反应中重要的保守调节因子和因子，与该申请人最近资助的Discovery拨款申请一致。这在科学上具有重要的基础意义，因为这些因素在酵母等低级生物和包括人类在内的高级系统之间通常是高度保守的。对这些过程的深入了解，不仅可以为了解在所有生命形式中发现的对压力和代谢过程的进化保守适应的本质提供有价值的信息，而且还可以阐明微生物有机体和高等有机体之间的分歧路径，为抗微生物化合物提供潜在的新靶点。对于第二个申请人，所要求的设备将主要用于培养酿酒酵母，以开展酵母PHD结构域蛋白的功能研究，该研究最近得到了NSERC的发现资助。本项目的目的是揭示细胞与不同酵母PHD结构域蛋白的相互作用以及这些相互作用对正常细胞过程的影响。该设备对于两个实验室项目的成功至关重要，因为约克没有合适的专用振动筛可用于拟议的研究。这种设备将提供给该部其他研究小组的成员，并将用于培训高素质的人员。

This application is submitted by two primary investigators at York University and requests funds for the purchase of two shaker incubators and accessories for the dedicated growth and study of the yeasts Schizosaccharomyces pombe and Saccharomyces cerevisiae. For the primary applicant, this equipment will be used specifically for the growth of S. pombe for the establishment of genetic screens searching for conserved regulators and factors important in cellular growth, metabolism and the response of cells to environmental stress, in agreement with this applicant's recently funded Discovery grant application. This is of fundamental scientific importance, as such factors are often highly conserved between lower organisms such as yeast and higher systems, including humans. Insight into such processes should not only provide valuable information into the nature of evolutionarily conserved adaptations to stress and metabolic processes found in all life forms, but could also illuminate paths of divergence between microbial organisms and higher organisms, providing potentially novel targets for anti-microbial compounds. For the secondary applicant, the requested equipment will be used primarily for growing S. cerevisiae to carry out the functional studies of the yeast PHD domain proteins, which was recently funded by an NSERC discovery grant. The objective of this project is to reveal the cellular interactions with different yeast PHD domain proteins and the effect of these interactions on normal cellular processes. This equipment will be essential to success of the projects of both laboratories, as there is no suitable and dedicated shaker available for the proposed research at York. This equipment will be made available to members of other research groups in the department and will be used in training of highly qualified personnel.