Development of closely-coupled nanostructures for advanced sensing and imaging
开发用于先进传感和成像的紧密耦合纳米结构
基本信息
- 批准号:RGPIN-2020-06073
- 负责人:
- 金额:$ 3.5万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2022
- 资助国家:加拿大
- 起止时间:2022-01-01 至 2023-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The proposed research program aims to develop closely-coupled nanoparticle assemblies as optical sensors for the unmet needs in biomedical research, healthcare and security. Sensing based on single plasmonic nanostructure is shown to be invaluable for elucidating biomolecular interactions in the microenvironment; however demonstrations have thus far been limited to proof-of-concept studies because of the inaccessibility of the customized research-grade instrumentation setups. The proposed work aims to overcome this drawback by designing bioprogrammable bottom-up self-assembled nanoparticle clusters. These plasmonic assemblies exhibit enhanced light scattering to enable the extrapolation of the optical response of thousands of sensors in parallel from darkfield microscopy images. The objectives are to develop and evaluate the sensing platform for bioanalysis down to the single-cell level, and to establish the capability of spatial mapping of molecular distribution and chemical gradient in the microenvironment. Specifically, the proposed research aims to develop new biomolecular linkers, novel multifunctional nanoparticles and large-area multiplexed sensing patch to reach the goals. In the first research area, we will design new biomolecular linkers based on nucleic acids and their hybrid conjugates with peptides for imparting responsivity of the plasmonic assemblies to biomarkers such as microRNA and proteins. The detection of biomolecules from single cells will be pursued to verify the applicability of the technology in analyzing the cellular distribution of biomarkers. In the second research area, we will develop a new class of nanoparticle assemblies comprising orthogonal optical properties of fluorescence and localized surface plasmon resonance. In combination with the newly developed biomolecular linkers, the multifunctional assemblies will be integrated into a robust large-area flexible sensing patch to enable multiplexed detection for field or point-of-care applications. The proposed sensing platform contrasts existing single-cell analytical technologies and molecular imaging techniques which require large instrumentations, centralized laboratory and sample preparation that is laborious and costly. By developing portable, economical and user-friendly sensing platform for analyses that otherwise could only be carried out in research settings, our work will help bridge the gap of fundamental research and practical applications.
拟议的研究计划旨在开发紧密耦合的纳米颗粒组件作为光学传感器,以满足生物医学研究,医疗保健和安全方面未满足的需求。基于单个等离子体纳米结构的传感被证明对于阐明微环境中的生物分子相互作用是非常宝贵的;然而,由于定制的研究级仪器设置的不可访问性,演示迄今为止仅限于概念验证研究。拟议的工作旨在通过设计生物可编程的自下而上的自组装纳米粒子簇来克服这一缺点。这些等离子体激元组件表现出增强的光散射,使外推的光学响应的数千个传感器并行从暗场显微镜图像。目标是开发和评估生物分析的传感平台,直至单细胞水平,并建立微环境中分子分布和化学梯度的空间映射能力。 具体而言,拟议的研究旨在开发新的生物分子连接器,新型多功能纳米颗粒和大面积多路传感贴片,以达到目标。在第一个研究领域,我们将设计基于核酸及其与肽的杂合缀合物的新生物分子连接体,用于赋予等离子体组装体对生物标志物如microRNA和蛋白质的响应性。将继续从单细胞中检测生物分子,以验证该技术在分析生物标志物的细胞分布中的适用性。在第二个研究领域,我们将开发一类新的纳米粒子组装体,包括荧光和局部表面等离子体共振的正交光学特性。结合新开发的生物分子连接器,多功能组件将被集成到一个强大的大面积柔性传感贴片中,以实现现场或护理点应用的多重检测。所提出的传感平台对比了现有的单细胞分析技术和分子成像技术,这些技术需要大型仪器,集中实验室和费力且昂贵的样品制备。通过开发便携式,经济和用户友好的传感平台,否则只能在研究环境中进行分析,我们的工作将有助于弥合基础研究和实际应用之间的差距。
项目成果
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Chen, Jennifer其他文献
CAGS AND ACS EVIDENCE BASED REVIEWS IN SURGERY. 40 Axillary dissection versus no axillary dissection in women with invasive breast cancer and sentinel node metastasis
- DOI:
10.1503/cjs.036011 - 发表时间:
2012-02-01 - 期刊:
- 影响因子:2.5
- 作者:
Latosinsky, Steve;Berrang, Tanya S.;Chen, Jennifer - 通讯作者:
Chen, Jennifer
Concerns of Hereditary Breast Cancer in Pregnancy and Lactation
- DOI:
10.1007/978-3-030-41596-9_17 - 发表时间:
2020-01-01 - 期刊:
- 影响因子:0
- 作者:
Chen, Jennifer;Prasath, Vishnu;Habibi, Mehran - 通讯作者:
Habibi, Mehran
Return to Work for Nurses With Hand Dermatitis
- DOI:
10.1097/der.0000000000000215 - 发表时间:
2016-09-01 - 期刊:
- 影响因子:5.2
- 作者:
Chen, Jennifer;Gomez, Pilar;Skotnicki, Sandra - 通讯作者:
Skotnicki, Sandra
SPONTANEOUS RESOLUTION OF A STAGE 3 MACULAR HOLE: Long-Term Follow-up With Optical Coherence Tomography.
- DOI:
10.1097/icb.0b013e31809ed990 - 发表时间:
2008-01-01 - 期刊:
- 影响因子:0
- 作者:
Chen, Jennifer;Lee, Lawrence;Vorster, Stephni - 通讯作者:
Vorster, Stephni
Pediatric Skull Fracture Characteristics Associated with the Development of Leptomeningeal Cysts in Young Children after Trauma: A Single Institution's Experience
- DOI:
10.1097/prs.0000000000006745 - 发表时间:
2020-05-01 - 期刊:
- 影响因子:3.6
- 作者:
Lopez, Joseph;Chen, Jennifer;Redett, Richard J. - 通讯作者:
Redett, Richard J.
Chen, Jennifer的其他文献
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{{ truncateString('Chen, Jennifer', 18)}}的其他基金
Dynamic Light Scattering Instrumentation for Materials Development
用于材料开发的动态光散射仪器
- 批准号:
RTI-2023-00419 - 财政年份:2022
- 资助金额:
$ 3.5万 - 项目类别:
Research Tools and Instruments
Development of closely-coupled nanostructures for advanced sensing and imaging
开发用于先进传感和成像的紧密耦合纳米结构
- 批准号:
RGPAS-2020-00050 - 财政年份:2022
- 资助金额:
$ 3.5万 - 项目类别:
Discovery Grants Program - Accelerator Supplements
Development of closely-coupled nanostructures for advanced sensing and imaging
开发用于先进传感和成像的紧密耦合纳米结构
- 批准号:
RGPAS-2020-00050 - 财政年份:2021
- 资助金额:
$ 3.5万 - 项目类别:
Discovery Grants Program - Accelerator Supplements
Development of closely-coupled nanostructures for advanced sensing and imaging
开发用于先进传感和成像的紧密耦合纳米结构
- 批准号:
RGPIN-2020-06073 - 财政年份:2021
- 资助金额:
$ 3.5万 - 项目类别:
Discovery Grants Program - Individual
Development of closely-coupled nanostructures for advanced sensing and imaging
开发用于先进传感和成像的紧密耦合纳米结构
- 批准号:
RGPIN-2020-06073 - 财政年份:2020
- 资助金额:
$ 3.5万 - 项目类别:
Discovery Grants Program - Individual
Development of closely-coupled nanostructures for advanced sensing and imaging
开发用于先进传感和成像的紧密耦合纳米结构
- 批准号:
RGPAS-2020-00050 - 财政年份:2020
- 资助金额:
$ 3.5万 - 项目类别:
Discovery Grants Program - Accelerator Supplements
Biomolecular-Mediated Plasmonic Sensors for Chemical and Biosensing in Complex Media
用于复杂介质中化学和生物传感的生物分子介导的等离子体传感器
- 批准号:
435664-2013 - 财政年份:2019
- 资助金额:
$ 3.5万 - 项目类别:
Discovery Grants Program - Individual
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