Critical need for absolute molecular weight determination on size exclusion chromatography system
尺寸排阻色谱系统绝对分子量测定的迫切需求
基本信息
- 批准号:RTI-2023-00316
- 负责人:
- 金额:$ 10.93万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Research Tools and Instruments
- 财政年份:2022
- 资助国家:加拿大
- 起止时间:2022-01-01 至 2023-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The size, or molecular weight, of a polymer is one of the most important factors in determining physical properties. Viscosity, degree of crystallinity, stiffness, bioavailability, conductivity, and other properties are all intimately associated with molecular weight. For example, for a given identical repeat unit of ethylene, polypropylene with a low molecular weight may be used as a plastic bag but polypropylene with ultrahigh high molecular weight may be used as anti-stab vests. Size exclusion chromatography (SEC) is a ubiquitous equipment to determine the molecular weight. The molecular weight is estimated by the time it takes for the polymer to elute from the column: the smaller the polymer, the more it interacts with the stationary solid phase of the column, the longer it takes to elute. Notably, typical methods widespread in the polymer research community and also at UofT inaccurately determine the molecular weight. Most researchers use a set of standard polymer samples with known molecular weight to create a calibration curve to correlate the time it takes for a polymer to elute and its known molecular weight. However, the time it takes to elute is intimately related to the identity of the polymer. For the same molecular weight, a polymer that is tightly coiled in a select solvent will elute at a different time that a polymer that is loosely coiled. Thus, the identity of the standard polymer samples used to create the calibration curve must match the identity of the polymer to be characterized. Since there are limited standards, often poly(styrene) or poly(methyl methacrylate) is used as a standard. Thus, the molecular weights determined by SEC calibrated against a standard is only a good estimated if the unknown polymer behaves like the standards. As new polymers are being developed at UofT, we are discovering that this estimation is no longer appropriate, where there are very large discrepancies. The requested SEC system coupled with a multi-angle light scattering technique, the first of its type at UofT and surrounding institutions to our knowledge, will enable the local polymer research community to determine absolute molecular weight. Moreover, the selected solvent system of chloroform is missing from UofT, which is the only solvent which solubilizes the new conjugated polymers developed by some of the UofT researchers. Also, it uses a quick analysis procedure and 85% less solvent per run than any system that is available on the market. The five co-PIs include Tran, Khan, Seferos, Winnik, and Bender, with more than 50 highly qualified personnel as potential users at the onset.
聚合物的大小或分子量是决定其物理性质的最重要因素之一。粘度、结晶度、硬度、生物利用度、电导率和其他性能都与分子量密切相关。例如,对于给定的相同重复单位的乙烯,低分子量的聚丙烯可用作塑料袋,而超高分子量的聚丙烯可用作防刺背心。粒径排除色谱法(SEC)是一种普遍存在的测定分子量的设备。分子量是通过聚合物从色谱柱中洗脱所需的时间来估计的:聚合物越小,它与色谱柱的固定固相相互作用越多,洗脱所需的时间就越长。值得注意的是,在聚合物研究界和UofT广泛使用的典型方法不能准确地确定分子量。大多数研究人员使用一组已知分子量的标准聚合物样品来创建校准曲线,以将聚合物洗脱所需的时间与其已知分子量相关联。然而,洗脱所需的时间与聚合物的特性密切相关。对于相同的分子量,在选定的溶剂中紧密盘绕的聚合物与松散盘绕的聚合物洗脱的时间不同。因此,用于创建校准曲线的标准聚合物样品的特性必须与待表征聚合物的特性相匹配。由于标准有限,通常使用聚(苯乙烯)或聚(甲基丙烯酸甲酯)作为标准。因此,如果未知聚合物的行为与标准相似,则根据标准校准的SEC确定的分子量仅是一个很好的估计。由于UofT正在开发新的聚合物,我们发现这种估计不再合适,因为存在很大的差异。据我们所知,UofT和周边机构首次采用了SEC系统和多角度光散射技术,这将使当地聚合物研究团体能够确定绝对分子量。此外,UofT缺少氯仿的溶剂体系,这是一些UofT研究人员开发的新型共轭聚合物的唯一溶剂。此外,它使用快速分析程序,每次运行比市场上任何系统都少85%的溶剂。五个合作项目包括Tran、Khan、Seferos、Winnik和Bender,一开始就有50多名高素质人员作为潜在用户。
项目成果
期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
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Tran, Helen其他文献
Extent of Follow-Up on Abnormal Cancer Screening in Multiple California Public Hospital Systems: A Retrospective Review.
- DOI:
10.1007/s11606-022-07657-4 - 发表时间:
2023-01 - 期刊:
- 影响因子:5.7
- 作者:
Khoong, Elaine C.;Rivadeneira, Natalie A.;Pacca, Lucia;Schillinger, Dean;Lown, David;Babaria, Palav;Gupta, Neha;Pramanik, Rajiv;Tran, Helen;Whitezell, Tyler;Somsouk, Ma;Sarkar, Urmimala - 通讯作者:
Sarkar, Urmimala
Vertebral osteomyelitis secondary to Streptococcus cristatus infection.
- DOI:
10.1016/j.heliyon.2023.e19616 - 发表时间:
2023-09 - 期刊:
- 影响因子:4
- 作者:
Tran, Helen;Ai, Angela;Gallardo-Huizar, Oscar E.;Kahn, Michael;Mathisen, Glenn - 通讯作者:
Mathisen, Glenn
Folding of a Single-Chain, Information-Rich Polypeptoid Sequence into a Highly Ordered Nanosheet
- DOI:
10.1002/bip.21590 - 发表时间:
2011-01-01 - 期刊:
- 影响因子:2.9
- 作者:
Kudirka, Romas;Tran, Helen;Zuckermann, Ronald N. - 通讯作者:
Zuckermann, Ronald N.
A Field Guide to Optimizing Peptoid Synthesis.
- DOI:
10.1021/acspolymersau.2c00036 - 发表时间:
2022-12-14 - 期刊:
- 影响因子:0
- 作者:
Clapperton, Abigail Mae;Babi, Jon;Tran, Helen - 通讯作者:
Tran, Helen
Hair Loss Profiles and Ritlecitinib Efficacy in Patients with Alopecia Areata: Post Hoc Analysis of the ALLEGRO Phase 2b/3 Study.
- DOI:
10.1007/s13555-023-00997-x - 发表时间:
2023-11 - 期刊:
- 影响因子:3.4
- 作者:
Thaci, Diamant;Tziotzios, Christos;Ito, Taisuke;Ko, Justin;Karadag, Ayse Serap;Fang, Hong;Edwards, Roger A.;Bonfanti, Gianluca;Wolk, Robert;Tran, Helen;Law, Ernest - 通讯作者:
Law, Ernest
Tran, Helen的其他文献
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{{ truncateString('Tran, Helen', 18)}}的其他基金
Macromolecular Bioelectronics Encoded for Biocompatibility, Self-assembly, and Degradability
编码生物相容性、自组装和可降解性的高分子生物电子学
- 批准号:
RGPIN-2021-03554 - 财政年份:2022
- 资助金额:
$ 10.93万 - 项目类别:
Discovery Grants Program - Individual
Macromolecular Bioelectronics Encoded for Biocompatibility, Self-assembly, and Degradability
编码生物相容性、自组装和可降解性的高分子生物电子学
- 批准号:
RGPIN-2021-03554 - 财政年份:2021
- 资助金额:
$ 10.93万 - 项目类别:
Discovery Grants Program - Individual
Macromolecular Bioelectronics Encoded for Biocompatibility, Self-assembly, and Degradability
编码生物相容性、自组装和可降解性的高分子生物电子学
- 批准号:
DGECR-2021-00343 - 财政年份:2021
- 资助金额:
$ 10.93万 - 项目类别:
Discovery Launch Supplement
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