L2M NSERC - Market assessment of Loop-Mediated Isothermal Amplification (LAMP) technology to inform quality control and microbe detection in baking, dairy, and other pathogens

L2M NSERC - 环介导等温扩增 (LAMP) 技术的市场评估，为烘焙、乳制品和其他病原体的质量控制和微生物检测提供信息

• 批准号: 576605-2022
• 负责人: Sabatinos, SarahSA
• 金额: $ 1.46万
• 依托单位: Ryerson University
• 依托单位国家: 加拿大
• 项目类别: Idea to Innovation
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=748522
• 关键词: L2M; NSERC; Market; assessment; Loop

Loop-mediated isothermal amplification (LAMP) is similar to polymerase chain reaction (PCR) in that it amplifies a target DNA to detect the presence of bacteria, viruses, or fungi. A version of PCR was highlighted in the COVID-19 pandemic for its ability to detect minute levels of virus in patient samples. Yet PCR has several disadvantages when applied to mass testing: it requires specialized training and expensive equipment and enzymes. Testing level is limited by machine capacity. Detecting a positive result requires specialized detection and analysis.LAMP is a method that performs the same function as PCR, but in a fast, efficient, and more cost-effective way. LAMP tests can be run without specialized equipment or training, and detection is by a colour change. LAMP sensitivity is excellent, and can detect microbial contamination from various surfaces. Using new methods of enzyme generation and purification, we are making a LAMP kit for the baking industry to avoid spoilage. This Lab2Market I2I Market Assessment will allow us to look at all the industries that we might impact: livestock, produce, surfaces, and biomedical applications. Our market assessment will also allow us to consider requirements of Canadian and American regulatory bodies including Health Canada, Agriculture and Agri-Food Canada, and the Food and Drug Administration (FDA), and consider packaging and distribution within various sectors.Our LAMP enzymes and kit components will provide an opportunity for immediate and high-level detection required by various industries with linked market considerations.

环介导等温扩增（LAMP）类似于聚合酶链反应（PCR），因为它扩增靶DNA以检测细菌，病毒或真菌的存在。在COVID-19大流行中，PCR的一个版本因其能够检测患者样本中微量病毒而受到重视。然而，PCR在应用于大规模检测时有几个缺点：它需要专门的培训和昂贵的设备和酶。测试水平受机器容量限制。检测阳性结果需要专门的检测和分析。LAMP是一种执行与PCR相同功能的方法，但快速，高效，更具成本效益。LAMP测试可以在没有专门设备或培训的情况下运行，并且通过颜色变化进行检测。LAMP灵敏度非常高，可以检测各种表面的微生物污染。使用新的酶生成和纯化方法，我们正在为烘焙行业制作LAMP试剂盒，以避免腐败。这个Lab 2 Market I2 I市场评估将使我们能够了解我们可能影响的所有行业：畜牧业，农产品，表面和生物医学应用。我们的市场评估还将使我们能够考虑加拿大和美国监管机构的要求，包括加拿大卫生部、加拿大农业和农业食品部以及美国食品和药物管理局（FDA），并考虑在各个行业的包装和分销。我们的LAMP酶和试剂盒组件将为具有相关市场考虑的各个行业提供即时和高水平检测的机会。