L2M NSERC - Market assessment of Loop-Mediated Isothermal Amplification (LAMP) technology to inform quality control and microbe detection in baking, dairy, and other pathogens

环介导的等温扩增（LAMP）类似于聚合酶链反应（PCR），它可以扩增目标DNA以检测细菌、病毒或真菌的存在。在2019冠状病毒病大流行中，一种PCR方法因能够检测患者样本中微量病毒而受到重视。然而，PCR应用于大规模检测时有几个缺点：它需要专门的培训和昂贵的设备和酶。测试水平受机器容量限制。检测阳性结果需要专门的检测和分析。LAMP是一种与PCR功能相同的方法，但具有快速、高效和更具成本效益的方法。LAMP测试可以在没有专门设备或培训的情况下进行，通过颜色变化来检测。LAMP灵敏度极好，可检测各种表面的微生物污染。使用新的酶生成和纯化方法，我们正在为烘焙行业制作LAMP套件，以避免变质。Lab2Market I2I市场评估将使我们能够查看我们可能影响的所有行业：畜牧业，农产品，表面和生物医学应用。我们的市场评估还将使我们能够考虑加拿大和美国监管机构的要求，包括加拿大卫生部、加拿大农业和农业食品部以及食品和药物管理局（FDA），并考虑各个部门的包装和分销。我们的LAMP酶和试剂盒组件将为各种行业提供即时和高水平的检测机会，并考虑到相关的市场因素。

Loop-mediated isothermal amplification (LAMP) is similar to polymerase chain reaction (PCR) in that it amplifies a target DNA to detect the presence of bacteria, viruses, or fungi. A version of PCR was highlighted in the COVID-19 pandemic for its ability to detect minute levels of virus in patient samples. Yet PCR has several disadvantages when applied to mass testing: it requires specialized training and expensive equipment and enzymes. Testing level is limited by machine capacity. Detecting a positive result requires specialized detection and analysis.LAMP is a method that performs the same function as PCR, but in a fast, efficient, and more cost-effective way. LAMP tests can be run without specialized equipment or training, and detection is by a colour change. LAMP sensitivity is excellent, and can detect microbial contamination from various surfaces. Using new methods of enzyme generation and purification, we are making a LAMP kit for the baking industry to avoid spoilage. This Lab2Market I2I Market Assessment will allow us to look at all the industries that we might impact: livestock, produce, surfaces, and biomedical applications. Our market assessment will also allow us to consider requirements of Canadian and American regulatory bodies including Health Canada, Agriculture and Agri-Food Canada, and the Food and Drug Administration (FDA), and consider packaging and distribution within various sectors.Our LAMP enzymes and kit components will provide an opportunity for immediate and high-level detection required by various industries with linked market considerations.