L2M NSERC - Market assessment of Loop-Mediated Isothermal Amplification (LAMP) technology to inform quality control and microbe detection in baking, dairy, and other pathogens

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Loop-mediated isothermal amplification (LAMP) is similar to polymerase chain reaction (PCR) in that it amplifies a target DNA to detect the presence of bacteria, viruses, or fungi. A version of PCR was highlighted in the COVID-19 pandemic for its ability to detect minute levels of virus in patient samples. Yet PCR has several disadvantages when applied to mass testing: it requires specialized training and expensive equipment and enzymes. Testing level is limited by machine capacity. Detecting a positive result requires specialized detection and analysis.LAMP is a method that performs the same function as PCR, but in a fast, efficient, and more cost-effective way. LAMP tests can be run without specialized equipment or training, and detection is by a colour change. LAMP sensitivity is excellent, and can detect microbial contamination from various surfaces. Using new methods of enzyme generation and purification, we are making a LAMP kit for the baking industry to avoid spoilage. This Lab2Market I2I Market Assessment will allow us to look at all the industries that we might impact: livestock, produce, surfaces, and biomedical applications. Our market assessment will also allow us to consider requirements of Canadian and American regulatory bodies including Health Canada, Agriculture and Agri-Food Canada, and the Food and Drug Administration (FDA), and consider packaging and distribution within various sectors.Our LAMP enzymes and kit components will provide an opportunity for immediate and high-level detection required by various industries with linked market considerations.