PROMA - PlatfoRm fOr Metabolites Analyses

PROMA - 代谢物分析平台

• 批准号: RTI-2023-00107
• 负责人: DesgagnéPenix, Isabel
• 金额: $ 10.87万
• 依托单位: Université du Québec à Trois-Rivières
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=748758
• 关键词: PROMA; PlatfoRm; Metabolites; Analyses

Plants and microalgae are important sources for new bioactive molecules, which have gained popularity over the years due to their enormous potential as antiviral, immunosuppressive, anti-oxidant, enzyme inhibitors, herbicide, and anti-microbial agents. Among them, specialized metabolites (SM) such as those found in medicinal plants are widely used for various applications and often produced in nature in the plants exposed to biotic and abiotic stress factors. Crude plant extracts usually contain complex mixture of different bioactive SM and efficient systems are necessary to screen those plant materials. The rapid and accurate detection of bioactive SM is crucial in phytochemical investigations. Efficient screening of plant extracts is performed using chromatographic methods such as high-performance liquid chromatography (HPLC) in combination with mass spectrometry (MS). HPLC-MS provides a new powerful approach for the rapid qualitative determination and identification of unknown SM in plant extracts through efficient separation capabilities of HPLC and exact structural characterization by MS from a small amount of plant sample extracts. The present grant application aims to support the development of a PlatfoRm fOr Metabolites Analyses hereafter referred to as PROMA. PROMA will support the diverse but complementary research programs on SM from microalgae and plant cells, which are led by the professors I. Desgagné-Penix (IDP), and T. Missihoun (TM) at Université du Québec à Trois-Rivières (UQTR). PROMA comprises a HPLC-MS system necessary for the NSERC-led research programs of the co-applicants, which address very distinct questions (plant metabolism and synthetic biology & plant response to stress), but the basic phytochemical analyses used to study these questions are similar. For example, PROMA will be used for untargeted and targeted metabolomic study, high-throughput screening, identification, and quantification of several different types of SM such as alkaloids, cannabinoids, fatty acids, and phenolic compounds that are present at traces level in samples. The PROMA platform will provide accurate, sensitive and specific data in terms of detection, identification and quantification about specialized metabolites for research involving various species of bioengineered microalgae cells, fungal cells, callus, and plant tissues. Additionally, PROMA will be used by additional researchers involved in organic chemistry, forensics, biomedical and environmental sciences to obtain top quality data. This will lead to important advances in these disciplines. In addition to these advances, a large number of students will be trained to perform basic studies assays using PROMA and to acquire highly transferable skills that are required for all biochemistry researchers; thus, making them as future scientists with competitive and high-tech caliber formation.

植物和微藻是新型生物活性分子的重要来源，近年来由于其在抗病毒、免疫抑制、抗氧化、酶抑制剂、除草剂和抗微生物等方面的巨大潜力而受到广泛关注。其中，在药用植物中发现的特化代谢物（specialized metabolites， SM）被广泛应用于各种领域，通常在自然界中植物受到生物和非生物胁迫因素的作用下产生。植物粗提物通常含有不同生物活性SM的复杂混合物，筛选这些植物材料需要高效的系统。在植物化学研究中，快速准确地检测生物活性SM是至关重要的。采用高效液相色谱（HPLC）和质谱（MS）相结合的色谱方法对植物提取物进行高效筛选。高效液相色谱-质谱技术通过高效液相色谱的高效分离和质谱的精确结构表征，为植物提取物中未知SM的快速定性鉴定提供了一种新的有力方法。目前的拨款申请旨在支持代谢物分析平台（以下简称PROMA）的开发。PROMA将支持从微藻和植物细胞中提取SM的多种但互补的研究项目，该项目由quit<s:1>三河流域大学（UQTR）的I. desgagn - penix （IDP）和T. Missihoun （TM）教授领导。PROMA包括一个高效液相色谱-质谱系统，这是nserc领导的共同申请者的研究项目所必需的，它解决了非常不同的问题（植物代谢和合成生物学&植物对胁迫的反应），但用于研究这些问题的基本植物化学分析是相似的。例如，PROMA将用于非靶向和靶向代谢组学研究，高通量筛选，鉴定和定量几种不同类型的SM，如样品中痕量存在的生物碱，大麻素，脂肪酸和酚类化合物。PROMA平台将为涉及多种生物工程微藻细胞、真菌细胞、愈伤组织和植物组织的研究提供准确、敏感和特定的代谢物检测、鉴定和定量数据。此外，PROMA将被有机化学、法医学、生物医学和环境科学领域的其他研究人员使用，以获得高质量的数据。这将导致这些学科的重要进展。除了这些进步之外，大量学生将接受培训，使用PROMA进行基础研究分析，并获得所有生物化学研究人员所需的高度可转移的技能；从而使他们成为具有竞争力的未来科学家和高科技人才的形成。