Modification and characterisation or surfaces and plasma processes for biotechnological applications

用于生物技术应用的表面和等离子体工艺的改性和表征

• 批准号: RGPIN-2021-02554
• 负责人: Laroche, Gaétan
• 金额: $ 3.35万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=753950
• 关键词: Modification; characterisation; surfaces; plasma; processes

One of my research program objectives is to develop plasma- based surface modification strategies at atmospheric pressure to precisely control the functionalization of biomaterials for further conjugation of bioactive molecules. This requires correlating the plasma characteristics with the resulting plasma -treated surface chemistry using appropriate experimental planning and mathematical modelling. Another objective of my research program is to mimic the extracellular matrix on the surface of biomaterials by decorating it with both nanometric and micrometric geometrical cues containing bioactive molecules that promote cell adhesion, migration, and differentiation. The first objective of my research program will center on correlating plasma characteristics and surface chemistry of functionalized or coated polymers in dielectric barrier discharge (DBD) atmospheric pressure plasmas. In the course of the present research program, FTIR emission and absorption spectra will be simultaneously recorded to characterize atmospheric plasmas in which gaseous precursors are not only consumed, but also converted into other chemical species. The quantitative information about the nature and concentration of the newly formed species (obtained through absorption spectroscopy) and energetic parameters  (obtained by emission spectroscopy) will be correlated with the plasma--treated material surface physicochemical properties using multivariate analysis. The second objective of my research program will be to mimic the extracellular matrix on the surface of biomaterials by decorating it with both nanometric and micrometric geometrical cues containing bioactive molecules that promote cell adhesion, migration, and differentiation. To further our understanding of the geometry and sizes of bioactive cues and that drive cell differentiation, we will engineer trifunctional biomaterials patterned surfaces with cell adhesion, cell migration, and cell differentiation molecules at both the nano and micro scales. To do that, we will first use electron beam nanolithography to conjugate nano areas of the surface with one of the three aforementioned types of peptides. Thereafter, this nanopatterned surface will be used for more classical microlithography, to pattern the two other types of bioactive molecules on patterned micro areas . At the end, the resulting surface will consist in a trifunctionalized surface which somewhat mimics the extracellular matrix by exposing geometrically organized biological cues at both the nano and micro scales to the cellular environment. These surfaces will be investigated as to their potential to promote human mesenchymal stem cell differentiation.

我的研究项目目标之一是开发大气压下基于等离子体的表面改性策略，以精确控制生物材料的功能化，从而进一步缀合生物活性分子。这需要使用适当的实验计划和数学建模将等离子体特性与所得的等离子体处理的表面化学相关联。我的研究计划的另一个目标是模仿生物材料表面上的细胞外基质，通过用含有促进细胞粘附、迁移和分化的生物活性分子的纳米和微米几何线索来装饰它。我的研究计划的第一个目标将集中在相关的等离子体特性和功能化或涂层聚合物在介质阻挡放电（DBD）大气压等离子体的表面化学。在本研究计划的过程中，FTIR发射和吸收光谱将被同时记录，以表征大气等离子体，其中气态前体不仅被消耗，而且还被转化为其他化学物质。新形成的物种的性质和浓度（通过吸收光谱法获得）和能量参数（通过发射光谱法获得）的定量信息将与等离子体处理的材料表面的物理化学性质，使用多变量分析。我的研究计划的第二个目标将是模仿生物材料表面上的细胞外基质，通过用纳米和微米几何线索装饰它，其中含有促进细胞粘附，迁移和分化的生物活性分子。为了进一步了解生物活性线索的几何形状和大小以及驱动细胞分化的因素，我们将在纳米和微米尺度上设计具有细胞粘附，细胞迁移和细胞分化分子的三功能生物材料图案化表面。为此，我们将首先使用电子束纳米光刻技术将表面的纳米区域与上述三种类型的肽之一结合。此后，这种纳米图案化的表面将用于更经典的微光刻，以在图案化的微区域上图案化另外两种类型的生物活性分子。最后，所得的表面将存在于三功能化表面中，其通过将纳米尺度和微米尺度的几何组织的生物线索暴露于细胞环境而在一定程度上模拟细胞外基质。将研究这些表面促进人间充质干细胞分化的潜力。