Melt electrowrite system for biofabrication of complex engineered tissues

用于复杂工程组织生物制造的熔体电写系统

• 批准号: RTI-2023-00475
• 负责人: Simmons, Craig
• 金额: $ 10.93万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=759695
• 关键词: Melt; electrowrite; system; biofabrication; complex

Our interdisciplinary team of engineers and scientists is at the forefront of of biomaterials and tissue engineering research that promises to deliver living tissue replacements and advanced lab models for drug discovery. However, this promise can only be realized if engineered tissues replicate the structural, compositional, and mechanical complexity of their native counterparts they seek to replace. To this end, recent advances in bioprinting methods and devices are making fabrication of complex biomimetic tissues a reality. However, we currently do not have access to the the state-of-the-art bioprinters we need to create complex tissues. This has slowed our progress and stifles translation of our discoveries and inventions, limiting their impact and value. In this proposal, we request a versatile bioprinter to address this urgent need. This system will enable multiple print modes, including melt electrowriting (MEW) alone or in combination with 3D hydrogel bioprinting. MEW is not available on any bioprinter in Toronto, and the fully integrated capabilities of the requested printer will be unique in Canada. This system will enable us to engineer complex multicellular, multimaterial functional tissues with biomimetic mechanical properties, with initial applications in heart valve, intervertebral disc, blood vessel, heart muscle, and airway tissue engineering. Dozens of trainees will receive expert technical training on this equipment, gaining interdisciplinary expertise in bioprinting, biomaterial science, mechanical testing, tissue engineering, and related applications that will prepare them to contribute to rapidly growing industries in Canada, including biotechnology, medical devices, and regenerative medicine.

我们的跨学科工程师和科学家团队处于生物材料和组织工程研究的最前沿，有望为药物发现提供活组织替代品和先进的实验室模型。然而，只有当工程组织复制了它们试图取代的天然对应物的结构、组成和机械复杂性时，这种承诺才能实现。为此，生物打印方法和设备的最新进展正在使复杂仿生组织的制造成为现实。然而，我们目前无法获得我们需要的最先进的生物打印机来创建复杂的组织。这减缓了我们的进步，扼杀了我们的发现和发明的转化，限制了它们的影响和价值。在本提案中，我们要求使用一种多功能生物打印机来满足这一紧迫需求。该系统将实现多种打印模式，包括单独的熔融电写入（MEW）或与3D水凝胶生物打印相结合。MEW在多伦多的任何生物打印机上都不可用，所请求的打印机的完全集成功能将在加拿大独一无二。该系统将使我们能够工程复杂的多细胞，多材料的功能组织与仿生力学性能，初步应用于心脏瓣膜，椎间盘，血管，心肌，气道组织工程。 数十名学员将接受有关该设备的专家技术培训，获得生物打印、生物材料科学、机械测试、组织工程和相关应用方面的跨学科专业知识，这将使他们做好准备，为加拿大快速发展的行业做出贡献，包括生物技术、医疗器械和再生医学。