DISSECTING SIGNALING PATHWAYS IN PLANT IMMUNITY

剖析植物免疫中的信号通路

基本信息

  • 批准号:
    RGPIN-2019-04046
  • 负责人:
  • 金额:
    $ 5.03万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Discovery Grants Program - Individual
  • 财政年份:
    2022
  • 资助国家:
    加拿大
  • 起止时间:
    2022-01-01 至 2023-12-31
  • 项目状态:
    已结题

项目摘要

Dissecting signaling pathways in plant immunity Microbial diseases are a major threat to global crop production, resulting in annual crop yield losses between 10 to 16%. Billions of dollars are spent each year on pesticides to protect crops from pathogens. These chemicals are often damaging to the environment and may also lead to the evolution of pesticide-resistant pathogens. Thus, safe and sustainable pathogen control methods are critical to the success of modern agriculture. One successful approach is to breed crops with disease resistance (R) genes, which mostly encode nucleotide-binding domain and leucine-rich repeats (NLR) immune receptors that can perceive specific pathogens and trigger strong defense responses. Typical plant NLRs are further classified into Toll/interleukin-1 receptor (TIR) or coil-coiled (CC) type depending on their corresponding N-termini. Despite NLR-mediated resistance being highly effective, its molecular mechanism remains unclear. The long-term goal of my lab is to understand NLR-mediated immunity at the molecular level and use that knowledge to develop methods for sustainable crop protection. During the past decade, we have established several autoimmune models and successfully used them to discover many novel components that contribute to plant immunity. We showed that nucleocytoplasmic trafficking, transcriptional regulation, NLR dimerization/oligomerization, protein modifications and RNA processing all play important roles in NLR-mediated immunity. We also uncovered an NLR degradation pathway that regulates the homeostasis of NLRs. In this current NSERC Discovery program of research, we will build on these established autoimmune models and use next-generation sequencing, CRISPR/Cas9 gene editing and proteomics methods to identify key missing players of NLR-mediated immunity and elucidate how they function together to mount a proper defense upon pathogen attack. In the next five years, we will focus on the following two specific objectives: 1.Characterize TIR-type NLR (TNL) mediated immune signaling pathways activated through the helper NLRs, ADR1s and NRG1s; and 2.Dissect plant immune signaling pathways that are dependent or independent of the defense hormone salicylic acid. The proposed research will not only enrich our understanding of how plants defend against microbial pathogens and train the next-generation of highly qualified diverse scientists, but will also provide new insight for designing novel strategies to protect the crop plants without reliance on pesticides. In addition, as NLR receptors are also present in mammals, our proposed studies on plant NLRs may shed light on how their animal counterparts function and could thus have potential health benefits.
微生物病害是全球农作物生产的主要威胁,每年造成农作物产量损失10%至16%。每年有数十亿美元用于杀虫剂,以保护农作物免受病原体的侵害。这些化学品往往对环境造成破坏,还可能导致抗药性病原体的进化。因此,安全和可持续的病原体控制方法对现代农业的成功至关重要。一种成功的方法是培育具有抗病(R)基因的作物,这些基因主要编码核苷酸结合结构域和富含亮氨酸重复序列(NLR)的免疫受体,这些受体可以感知特定的病原体并引发强烈的防御反应。典型的植物NLR根据其相应的N-末端进一步分为Toll/白细胞介素-1受体(TIR)或卷曲螺旋(CC)类型。尽管NLR介导的抗性非常有效,但其分子机制仍不清楚。我实验室的长期目标是在分子水平上了解NLR介导的免疫,并利用这些知识开发可持续作物保护的方法。在过去的十年中,我们已经建立了几个自身免疫模型,并成功地利用它们来发现许多新的成分,有助于植物免疫。我们发现,核质运输,转录调控,NLR二聚化/寡聚化,蛋白质修饰和RNA加工都在NLR介导的免疫中发挥重要作用。我们还发现了一个NLR降解途径,调节NLR的稳态。在目前的NSERC发现研究计划中,我们将建立在这些已建立的自身免疫模型的基础上,并使用下一代测序,CRISPR/Cas9基因编辑和蛋白质组学方法来识别NLR介导的免疫的关键缺失参与者,并阐明它们如何共同发挥作用,以在病原体攻击时进行适当的防御。 在未来的五年里,我们将致力于以下两个具体目标:1.表征通过辅助NLR、ADR 1和NRG 1激活的TIR型NLR(TNL)介导的免疫信号通路; 2.剖析依赖或不依赖于防御激素水杨酸的植物免疫信号通路。拟议的研究不仅将丰富我们对植物如何防御微生物病原体的理解,并培养下一代高素质的多样化科学家,还将为设计新策略提供新的见解,以保护作物植物而不依赖农药。此外,由于NLR受体也存在于哺乳动物中,我们提出的对植物NLR的研究可能会揭示其动物对应物的功能,从而可能具有潜在的健康益处。

项目成果

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Li, Xin其他文献

Plasma metabolomic characterization of premature ovarian insufficiency.
  • DOI:
    10.1186/s13048-022-01085-y
  • 发表时间:
    2023-01-05
  • 期刊:
  • 影响因子:
    4
  • 作者:
    Zhou, Xing-Yu;Li, Xin;Zhang, Jun;Li, Ying;Wu, Xiao-Min;Yang, Yi-Zhen;Zhang, Xiao-Fei;Ma, Lin-Zi;Liu, Yu-Dong;Wang, Zhe;Chen, Shi-Ling
  • 通讯作者:
    Chen, Shi-Ling
Sarcoidosis misdiagnosed as malignant tumors: a case report
  • DOI:
    10.1186/s12957-015-0748-6
  • 发表时间:
    2015-12-12
  • 期刊:
  • 影响因子:
    3.2
  • 作者:
    Li, Zuosheng;Li, Xin;Chen, Jun
  • 通讯作者:
    Chen, Jun
Nosocomial spread of OXA-232-producing Klebsiella pneumoniae ST15 in a teaching hospital, Shanghai, China
  • DOI:
    10.1186/s12866-019-1609-1
  • 发表时间:
    2019-10-28
  • 期刊:
  • 影响因子:
    4.2
  • 作者:
    Li, Xin;Ma, Wei;Li, Boan
  • 通讯作者:
    Li, Boan
Immunogenicity Analysis of a Novel Subunit Vaccine Candidate Molecule-Recombinant L7/L12 Ribosomal Protein of Brucella suis
  • DOI:
    10.1007/s12010-016-2076-x
  • 发表时间:
    2016-08-01
  • 期刊:
  • 影响因子:
    3
  • 作者:
    Du, Zhi-Qiang;Li, Xin;Wang, Jian-Ying
  • 通讯作者:
    Wang, Jian-Ying
Assimilating passive microwave remote sensing data into a land surface model to improve the estimation of snow depth
将被动微波遥感数据同化到地表模型中以改进雪深的估计
  • DOI:
    10.1016/j.rse.2013.12.009
  • 发表时间:
    2014-03
  • 期刊:
  • 影响因子:
    13.5
  • 作者:
    Che, Tao;Li, Xin;Jin, Rui;Huang, Chunlin
  • 通讯作者:
    Huang, Chunlin

Li, Xin的其他文献

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{{ truncateString('Li, Xin', 18)}}的其他基金

Plant immunity
植物免疫
  • 批准号:
    CRC-2021-00336
  • 财政年份:
    2022
  • 资助金额:
    $ 5.03万
  • 项目类别:
    Canada Research Chairs
A high-performance liquid chromatography (HPLC) system for analyzing plant defense hormone salicylic acid
用于分析植物防御激素水杨酸的高效液相色谱 (HPLC) 系统
  • 批准号:
    RTI-2023-00119
  • 财政年份:
    2022
  • 资助金额:
    $ 5.03万
  • 项目类别:
    Research Tools and Instruments
DISSECTING SIGNALING PATHWAYS IN PLANT IMMUNITY
剖析植物免疫中的信号通路
  • 批准号:
    RGPIN-2019-04046
  • 财政年份:
    2021
  • 资助金额:
    $ 5.03万
  • 项目类别:
    Discovery Grants Program - Individual
PRoTECT - Plant Responses To Eliminate Critical Threats: An NSERC-CREATE-DFG-IRTG joint training program to train the next generation of "Plant Doctors"
PROTECT - 消除严重威胁的植物反应:NSERC-CREATE-DFG-IRTG 联合培训计划,旨在培训下一代“植物医生”
  • 批准号:
    509257-2018
  • 财政年份:
    2021
  • 资助金额:
    $ 5.03万
  • 项目类别:
    Collaborative Research and Training Experience
PRoTECT - Plant Responses To Eliminate Critical Threats: An NSERC-CREATE-DFG-IRTG joint training program to train the next generation of "Plant Doctors"
PROTECT - 消除严重威胁的植物反应:NSERC-CREATE-DFG-IRTG 联合培训计划,旨在培训下一代“植物医生”
  • 批准号:
    509257-2018
  • 财政年份:
    2020
  • 资助金额:
    $ 5.03万
  • 项目类别:
    Collaborative Research and Training Experience
DISSECTING SIGNALING PATHWAYS IN PLANT IMMUNITY
剖析植物免疫中的信号通路
  • 批准号:
    RGPIN-2019-04046
  • 财政年份:
    2020
  • 资助金额:
    $ 5.03万
  • 项目类别:
    Discovery Grants Program - Individual
DISSECTING SIGNALING PATHWAYS IN PLANT IMMUNITY
剖析植物免疫中的信号通路
  • 批准号:
    RGPIN-2019-04046
  • 财政年份:
    2019
  • 资助金额:
    $ 5.03万
  • 项目类别:
    Discovery Grants Program - Individual
PRoTECT - Plant Responses To Eliminate Critical Threats: An NSERC-CREATE-DFG-IRTG joint training program to train the next generation of "Plant Doctors"
PROTECT - 消除严重威胁的植物反应:NSERC-CREATE-DFG-IRTG 联合培训计划,旨在培训下一代“植物医生”
  • 批准号:
    509257-2018
  • 财政年份:
    2019
  • 资助金额:
    $ 5.03万
  • 项目类别:
    Collaborative Research and Training Experience
PRoTECT - Plant Responses To Eliminate Critical Threats: An NSERC-CREATE-DFG-IRTG joint training program to train the next generation of "Plant Doctors"
PROTECT - 消除严重威胁的植物反应:NSERC-CREATE-DFG-IRTG 联合培训计划,旨在培训下一代“植物医生”
  • 批准号:
    509257-2018
  • 财政年份:
    2018
  • 资助金额:
    $ 5.03万
  • 项目类别:
    Collaborative Research and Training Experience
Using an Autoimmune Model to Dissect Plant Immunity
使用自身免疫模型剖析植物免疫
  • 批准号:
    RGPIN-2014-05384
  • 财政年份:
    2018
  • 资助金额:
    $ 5.03万
  • 项目类别:
    Discovery Grants Program - Individual

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相似海外基金

CAREER: Dissecting the cellular pathways and signaling networks orchestrating plant defense responses and their interplay with bacterial virulence factors
职业:剖析细胞通路和信号网络,协调植物防御反应及其与细菌毒力因子的相互作用
  • 批准号:
    2332080
  • 财政年份:
    2023
  • 资助金额:
    $ 5.03万
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    Continuing Grant
Dissecting the interactions between TCR signaling strength and inhibitory pathways
剖析 TCR 信号强度和抑制途径之间的相互作用
  • 批准号:
    10649675
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    2022
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Dissecting the interactions between TCR signaling strength and inhibitory pathways
剖析 TCR 信号强度和抑制途径之间的相互作用
  • 批准号:
    10895664
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  • 资助金额:
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  • 项目类别:
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    10507695
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Dissecting Rotavirus Viroporin and Enterotoxin Calcium Signaling Pathways
剖析轮状病毒病毒孔蛋白和肠毒素钙信号通路
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    10372424
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DISSECTING SIGNALING PATHWAYS IN PLANT IMMUNITY
剖析植物免疫中的信号通路
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    RGPIN-2019-04046
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    2021
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    $ 5.03万
  • 项目类别:
    Discovery Grants Program - Individual
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    562252-2021
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    2021
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DISSECTING SIGNALING PATHWAYS IN PLANT IMMUNITY
剖析植物免疫中的信号通路
  • 批准号:
    RGPIN-2019-04046
  • 财政年份:
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  • 资助金额:
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