Improving Aspects of In-Vivo Magnetic Resonance Spectroscopy Metabolite and Fat Quantification

体内磁共振波谱代谢物和脂肪定量的改进

• 批准号: RGPIN-2018-04730
• 负责人: Yahya, Atiyah
• 金额: $ 3.5万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=761831
• 关键词: Improving; Aspects; Vivo; Magnetic; Resonance

Magnetic Resonance Spectroscopy (MRS) is a non-invasive (does not require a surgical procedure) methodology that can measure levels of relevant body and brain substances involved in metabolism (metabolites). It uses an MRI (magnetic resonance imaging) unit. The objective of the research program is to improve MRS quantification of relevant brain metabolites and body fat composition at the human field strength of 3 T and the stronger magnetic field strength of 9.4 T, often used in pre-clinical research on animals. Both MRI units are at the Cross Cancer Institute (Edmonton's cancer hospital). MRS techniques that enable improved determination of unsaturated fat (type of fat with at least one double bond in its structure) and omega-3 fat levels in adipose tissue, such as bone marrow and abdominal fat tissue will be developed for human (3 T) and animal studies (9.4 T). The measures are relevant because levels of fat unsaturation have been shown to change due to diseases such as osteoporosis, obesity, liver disease and cancer. In addition, possible changes in omega-3 content of breast tissue may be associated with breast cancer risk. Levels of omega-3 fatty acids are low in body fat tissue (about 1 2 %) and therefore, its detection with MRS is difficult, a challenge we aim to overcome. Our long term goals involve applying the developed methods to correlate fat composition with cancer risk, tumour types and treatment response in humans and animal (rodent) models of disease. In addition, MRS techniques that allow for improved measures of brain metabolism will be designed and implemented at 9.4 T. Specifically, improved quantification of dynamic measures (changes that occur during metabolism) of glutamate, glutamine and GABA (relevant brain metabolites) will be achieved to improve metabolism assessment in rodents. Our long term goals involve utilizing the developed methodologies to improve our understanding of brain tumour metabolism (in rodent models) and its response to treatment. Another long term goal is to investigate correlations between measures obtained with MRS and PET (Positron Emission Tomography), an imaging methodology that uses radioactive tracers. Knowledge obtained from the animal studies can be translated to human studies on the new PET/MRI unit at the Cross Cancer Institute. The developed techniques will also be of benefit to other MRS studies being conducted at 3 T and at 9.4 T in Canada, where brain metabolism or fat levels are being assessed. The methods will provide the means to address metabolite and fat quantification challenges encountered with MRS.

磁共振波谱（MRS）是一种非侵入性（不需要外科手术）方法，可以测量参与代谢的相关身体和大脑物质（代谢物）的水平。 它使用MRI（磁共振成像）单元。 该研究计划的目的是在人体磁场强度为3 T和更强的磁场强度为9.4 T的条件下，改善相关脑代谢物和体脂成分的MRS定量，常用于动物临床前研究。 这两个MRI单位都在交叉癌症研究所（埃德蒙顿的癌症医院）。 将开发MRS技术用于人体（3 T）和动物研究（9.4 T），该技术能够改进脂肪组织（如骨髓和腹部脂肪组织）中不饱和脂肪（结构中至少有一个双键的脂肪类型）和ω-3脂肪水平的测定。 这些措施是相关的，因为脂肪不饱和度的水平已被证明会因骨质疏松症，肥胖症，肝病和癌症等疾病而发生变化。 此外，乳腺组织中omega-3含量的可能变化可能与乳腺癌风险有关。 在身体脂肪组织中，ω-3脂肪酸的水平很低（约12%），因此，用MRS检测它是困难的，这是我们要克服的挑战。 我们的长期目标包括应用开发的方法将脂肪组成与人类和动物（啮齿动物）疾病模型中的癌症风险，肿瘤类型和治疗反应相关联。 此外，将在9.4 T下设计和实施允许改善脑代谢测量的MRS技术。 具体而言，将实现谷氨酸盐、谷氨酰胺和GABA（相关脑代谢物）的动态测量（代谢期间发生的变化）的改进量化，以改善啮齿动物的代谢评估。 我们的长期目标包括利用开发的方法来提高我们对脑肿瘤代谢（在啮齿动物模型中）及其对治疗反应的理解。 另一个长期目标是研究MRS和PET（正电子发射断层扫描）（一种使用放射性示踪剂的成像方法）测量结果之间的相关性。 从动物研究中获得的知识可以在Cross Cancer Institute的新PET/MRI单元上转化为人类研究。 开发的技术也将有利于在加拿大进行的其他MRS研究在3 T和9.4 T，其中大脑代谢或脂肪水平进行评估。 该方法将提供解决MRS遇到的代谢物和脂肪定量挑战的方法。