Improving the Commercial Value of Anti-Fusarium Probiotics in Corn and Wheat By Rapid Breeding

加拿大西部的农民有机会出口更多的小麦，东部的农民有机会出售更多的玉米。挑战之一是由真菌病原体家族引起的谷物疾病，即禾谷镰刀菌（Fusarium graminearum sensu scricto）（Fgss），其每年给加拿大农民造成5千万至3亿美元的损失。Fgss降低产量，但更糟糕的是，分泌危险的毒素进入谷物。Fgss的爆发正在恶化，也许是由于气候变化。目前的杀真菌剂和最好的作物遗传学结合在一起，在严重爆发期间不能完全控制疾病。一个有希望的解决方案是堆叠第三种解决方案：益生菌。就像人类从益生菌中受益一样，作物也可以。将抗Fgss益生菌（生物防治剂）添加到杀真菌剂喷雾剂中可以完全抑制这些疾病，因为它们具有互补的作用模式。一种益生菌可以取代50%的杀真菌剂，但可以作为一种后期喷雾剂一起提供，以节省拖拉机/燃料成本，这将为自己买单。我们有6种来自小麦/玉米的安全，有前途的晚季细菌益生菌，可以对抗Fg，其中三种已经在最初的田间试验中显示出功效。它们是独特的，因为它们预先适应Fgss传播的玉米/小麦组织。为了向前迈进，私营部门告诉我们，益生菌必须非常有效，在不同环境中保持一致，耐受复杂的杀菌剂配方，并可申请专利。我们已经开发了新的育种策略（FieldAdapt，Enhanced seek-and-kill）来进化益生菌以克服这些挑战。

There is an opportunity for Western Canadian farmers to export more wheat, and for Eastern Canadian farmers to sell more corn. One of the challenges are grain diseases caused by the fungal pathogen family, Fusarium graminearum sensu scricto (Fgss) which causes $50-$300 million in losses annually to Canadian farmers. Fgss decreases yield, but worse, secrete dangerous toxins into the grain. Fgss outbreaks are worsening, perhaps due to climate change. The current fungicides and best crop genetics, together, do not fully control the disease during severe outbreaks. One promising solution is to stack a third solution: probiotics. Just as humans benefit from probiotics, so can crops. Adding anti-Fgss probiotics (biocontrol agents) to fungicide sprays could completely suppress these diseases, as they have complementary modes of action. A probiotic that can replace 50% of the fungicide, but be delivered together as a late-season-spray to save tractor/fuel costs, would pay for itself. We have 6 safe, promising, late-season bacterial probiotics from wheat/corn that combat Fg, three of which have already shown efficacy in initial field trials. They are unique, because they are pre-adapted to the corn/wheat tissues through which Fgss spreads. To move forward, the private sector has told us that probiotics must be very potent, consistent across environments, tolerate complex fungicide formulations and be patentable. We have developed novel breeding strategies (FieldAdapt, Enhanced seek-and-kill) to evolve the probiotics to overcome these challenges.