Hormonal Regulation of Feeding, Metabolism, Growth and Reproduction in Fish

鱼类摄食、代谢、生长和繁殖的激素调节

• 批准号: RGPIN-2022-04293
• 负责人: Unniappan, Suraj
• 金额: $ 4.74万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=766003
• 关键词: Hormonal; Regulation; Feeding; Metabolism; Growth

The long-term goal of my discovery research program is to create new knowledge through the characterization of novel hormones that regulate metabolism, growth and reproduction in fish. In the past five years, we discovered the biological actions of numerous hormones. Among them, nesfatin-1 and nesfatin-1-like peptide (Nlp) are two multifunctional peptides that were found to regulate feeding, metabolism, growth and reproduction in fish. In addition, three growth factors that regulate feeding and metabolism were characterized. While nesfatin-1 and Nlp have multiple biological actions, whether they are essential to maintain fish physiology is unknown. The mechanism of action of nesfatin-1 and Nlp is poorly understood. Whether growth factors regulate reproduction and growth in fish is unclear. This proposal builds on our original discoveries, and it is a logical and feasible extension of our research to date. It aims to conduct the next set of studies to fill several knowledge gaps on these important regulators in fish. Under objective 1, using genetic tools, we will delete nesfatin-1 and Nlp genes in zebrafish and determine how critical these two peptides are in maintaining metabolic homeostasis, growth and reproduction. It is anticipated that the loss of one or both peptides lead to disturbed metabolism, growth and reproduction. In objective 2, the mechanism of action of nesfatin-1 and Nlp will also be determined using pharmacological agents and genetic tools that can tease out specific receptors and pathways. We believe that a cell surface receptor and dedicated intracellular signals are important for nesfatin-1 and Nlp action. Objective 3 will explore the role of growth factors in regulating growth and reproduction will be studied by measuring the effects of these peptides on gamete development, ovarian follicle maturation, growth, body weight and major hormones that regulate these processes. We expect these previously uncharacterized growth factors to stimulate both growth and reproduction and the hormonal milieu that supports it in zebrafish. The novel fundamental knowledge created though this research will help redefine and update our current understanding of fish biology. It could be beneficial by helping learn how these hormones regulate important processes in other species including domestic animals. The outcomes of this program are expected to help develop new strategies to enhance fish growth and reproduction in aquaculture, thus contributing to sustainability and food security. This research provides remarkable opportunities for training the next generation of young researchers, especially women and those underrepresented in science. They will contribute to the pool of highly qualified personnel required by Canada's workforce and economy. Overall, the proposed research program will generate considerable new knowledge while contributing indirectly, yet significantly to the economy and sustainability efforts in Canada.

我的发现研究计划的长期目标是通过描述调节鱼类新陈代谢，生长和繁殖的新型激素来创造新的知识。在过去的五年里，我们发现了许多激素的生物作用。其中，nesfatin-1和nesfatin-1-like peptide（Nlp）是两种多功能肽，对鱼类的摄食、代谢、生长和繁殖具有调节作用。此外，三个生长因子，调节摄食和代谢的特点。虽然nesfatin-1和Nlp具有多种生物学作用，但它们是否对维持鱼类生理机能至关重要尚不清楚。nesfatin-1和Nlp的作用机制知之甚少。生长因子是否调节鱼类的繁殖和生长尚不清楚。这一建议建立在我们最初的发现基础上，是我们迄今为止研究的一个合乎逻辑和可行的延伸。它旨在进行下一组研究，以填补鱼类中这些重要调节剂的几个知识空白。在目标1下，使用遗传工具，我们将删除斑马鱼中的nesfatin-1和Nlp基因，并确定这两种肽在维持代谢稳态，生长和繁殖中的重要性。预期一种或两种肽的损失导致代谢、生长和繁殖紊乱。在目标2中，nesfatin-1和Nlp的作用机制也将使用可以梳理出特定受体和途径的药理学试剂和遗传工具来确定。我们认为，细胞表面受体和专用的细胞内信号是nesfatin-1和NLP行动的重要。目的3将探索生长因子在调节生长和生殖中的作用，通过测量这些肽对配子发育、卵泡成熟、生长、体重和调节这些过程的主要激素的影响来研究。我们期望这些以前未表征的生长因子刺激生长和繁殖以及支持斑马鱼生长和繁殖的激素环境。通过这项研究创造的新的基础知识将有助于重新定义和更新我们目前对鱼类生物学的理解。它可以帮助了解这些激素如何调节其他物种（包括家畜）的重要过程。该计划的成果预计将有助于制定新的战略，以提高水产养殖中鱼类的生长和繁殖，从而促进可持续性和粮食安全。这项研究为培养下一代年轻研究人员，特别是妇女和科学界代表性不足的人提供了极好的机会。他们将为加拿大的劳动力和经济所需的高素质人才库做出贡献。总的来说，拟议的研究计划将产生相当多的新知识，同时有助于间接，但显着的经济和可持续发展的努力在加拿大。