Canadian bats and their viruses: From benevolent relationships to spillover to other species

加拿大蝙蝠及其病毒：从仁慈关系到蔓延到其他物种

• 批准号: RGPIN-2018-04127
• 负责人: Misra, Vikram
• 金额: $ 6.12万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=766042
• 关键词: Canadian; bats; their; viruses; benevolent

Several viruses, that appear to cause no overt illness in their natural bat hosts, have spilled over into other mammals causing serious and often fatal disease. These include viruses that cause Severe Acute Respiratory Syndrome (SARS), Middle East Respiratory Syndrome (MERS); Marburg and Ebola haemorrhagic disease; Hendra, and Nipah respiratory and neurological disease, and porcine epidemic diarrhea (PED). While there are many studies on the interaction of these viruses with their spillover hosts or surrogate laboratory animals, there is little information on the relationship of these or similar viruses with their natural bat hosts. We therefore do not know the adaptations these viruses and their bat hosts have undergone during their co-evolution towards relatively benign relationships. There is also little information on how environmental and other factors alter the benign bat-virus relationship leading to spillover to other species. To fill these important knowledge gaps, the long-term goal of our laboratory is to define the relationship between two species of common Canadian bats and their viruses, and factors that disrupt this normally benign relationship leading to increased virus replication. Although interactions between Henipah viruses in their fruit-eating bat hosts have been studied, our work is the only known attempt to examine natural virus-host relationships in insectivorous bats. We have identified and characterized a unique coronavirus (related to SARS, MERS and PED causing viruses) and a herpes virus that naturally infect two Canadian bat species. Many, if not most bats, appear to be persistently infected with these viruses with limited virus replication and no overt signs of pathology or illness. Our preliminary studies suggest that secondary infection with a lethal fungus causes a large increase in virus load in the bats, potentially leading to spillover. Our strategy to define bat-virus relationships will be to use broad and deep information on gene expression in bats to form hypotheses on how bats respond to fungal and viral infection, test these hypotheses in cell culture models and then, in collaborative studies with bat biologists, validate the results in field samples. Our studies will not only provide fundamental information about virus-host relationships in these specialized mammals, but will also give clues about mitigating the serious consequences that often accompany viral spillover from bats to humans and other species.

有几种病毒，在它们的自然宿主蝙蝠身上似乎没有引起明显的疾病，但已经蔓延到其他哺乳动物身上，引起严重的，往往是致命的疾病。这些病毒包括引起严重急性呼吸系统综合征（SARS）、中东呼吸系统综合征（MERS）、马尔堡和埃博拉出血热、亨德拉和尼帕呼吸系统和神经系统疾病以及猪流行性腹泻（PED）的病毒。虽然有许多关于这些病毒与其溢出宿主或替代实验室动物的相互作用的研究，但关于这些或类似病毒与其天然蝙蝠宿主的关系的信息很少。因此，我们不知道这些病毒和它们的蝙蝠宿主在朝着相对良性的关系共同进化的过程中所经历的适应。关于环境和其他因素如何改变蝙蝠-病毒的良性关系，从而导致向其他物种蔓延的信息也很少。为了填补这些重要的知识空白，我们实验室的长期目标是确定两种常见的加拿大蝙蝠和它们的病毒之间的关系，以及破坏这种正常良性关系导致病毒复制增加的因素。虽然亨尼帕病毒在其食果蝙蝠宿主之间的相互作用已经研究，我们的工作是唯一已知的尝试，以研究自然病毒宿主关系的食虫蝙蝠。我们已经确定并表征了一种独特的冠状病毒（与SARS，MERS和PED相关）和一种疱疹病毒，它们自然感染两种加拿大蝙蝠物种。许多蝙蝠（如果不是大多数蝙蝠的话）似乎持续感染这些病毒，病毒复制有限，没有明显的病理或疾病迹象。 我们的初步研究表明，致命真菌的二次感染会导致蝙蝠体内病毒载量大幅增加，可能导致溢出。我们定义蝙蝠-病毒关系的策略将是利用蝙蝠基因表达的广泛而深入的信息，形成蝙蝠如何应对真菌和病毒感染的假设，在细胞培养模型中测试这些假设，然后与蝙蝠生物学家合作研究，验证现场样本的结果。我们的研究不仅将提供有关这些专门哺乳动物中病毒-宿主关系的基本信息，而且还将提供有关减轻病毒从蝙蝠传播到人类和其他物种的严重后果的线索。