口蹄疫是危害反刍兽最为严重的烈性传染病之一。发展中国家主要依赖于免疫接种而控制本病的蔓延。然而在防控FMDV的实际工作中，病毒多种血清型问题难以解决。本项目分别对A、O和Asia I型FMDV 的VP1抗原的差异性显著的表位肽进行分析，并将其嵌入到短小乳杆菌的S-层蛋白纳米级晶格中，构建2套多型表位疫苗载体系统pGEX-multi-EpitopeSLP和pNZ-multi-EpitopeSLP。应用SDS-PAGE、Western blotting及IFAT或流式细胞仪分析等方法鉴定S-层蛋白中嵌入的多表位。疫苗按既定方案免疫BALB/c小鼠，采用ELISA方法检测VP1抗原特异性抗体反应；从脾脏淋巴细胞中，应用定量PCR等方法检测IL-2、IL-4和IFN-γ等细胞因子水平；经脾脏淋巴细胞增殖试验评价TH或CD8T细胞介导的免疫反应。本研究进一步研制FMDV多型表位疫苗奠定基础。

FMD is one of the most serious deadly infectious diseases which is harmful to ruminants. Developing countries mainly rely on immunization vaccination to control the spread of this disease. However, in the practical work of the prevention and control of FMDV , the problem of virus serotypes is difficult to solve. This project respectively analyzed epitope peptides with significant differences of VP1 antigens of A、O and Asia I type FMDV.Then it was embedded into the S-layer protein nanometer lattice of Lactobacillus brevis,so two sets of polymorphic epitope vaccine vectors of pGEX-multi-Epitope SLP and pNZ-multi-EpitopeSLP were constructed.The multi-epitope embedded in S-layer protein was identified by SDS-PAGE, Western blotting and IFAT or flow cytometry analysis.BALB/c mice were immuned by the vaccines according to the established program,and the specific antibody response of VP1 antigens was detected by ELISA;The cytokines levels such as IL-2, IL-4 and IFN-γ were detected by quantitative PCR in spleen lymphocytes;Spleen lymphocyte proliferation test could evaluate TH or CD8 T cell-mediated immune response.This study laid a foundation for the further development of the FMDV multi-epitope vaccine.