基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)近年来在小分子分析领域的应用逐渐受到重视，然而由于低分子量区域的背景干扰和检测重现性的限制，MALDI-MS对小分子的定性定量能力尚显不足。富勒烯化学是研究以C60为代表的化学分支，目前已可以按照实际需要高效且定向地合成一系列功能化的C60衍生物，同时C60自身也可作为MALDI-MS的基质。本项目拟通过合成一系列以C60为基本结构单元的标记试剂，作为生物活性小分子的MALDI-MS“反应性基质”和质量位移试剂，并提供廉价易得的内标，从而解决MALDI-MS在小分子分析中存在的背景干扰和重现性差的问题，最终建立疾病相关的生物活性小分子准确、快速、经济的C60标记-MALDI-MS定量分析方法。本项目发展的以C60标记试剂作为MALDI-MS“反应性基质”的策略，有望使MALDI-MS成为小分子分析的高通量检测手段。

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has attracted great attentions in small molecule (<1000 Da) analysis recently due to its high throughput, high sensitivity and low sample consumption. However, matrix signals in low m/z range and poor reproducibility of MALDI-MS signals hampered its application to the qualitative and quantitative analysis of small molecules. Fullerene chemistry is a field of organic chemistry devoted to the chemical properties of C60 related macromolecules. Up to now, the functionalization of C60 has become mature and directional; people can synthesize C60 derivatives as desired. Moreover, C60 itself can serve as MALDI-MS matrix. Thanks to the properties of C60, the current project aims to design and synthesize a series of C60 derivatives that can act as reactive matrices to react with small molecules and move the molecular weight of target compounds into the high m/z region. By applying a pair of C60 homolog labeling reagents to derive small molecules in real sample and standard solution respectively, the matrix effect in low m/z region could be circumvented, the MALDI-MS signal variations could be corrected and also no additional matrix is required owing to the great laser desorption/ionization property of C60 derivatives themselves. Thus, a fast, economic and reliable qualitative and quantitative analysis of bioactive small molecules could be developed. The proposed strategy based on C60 derivatives as reactive matrices of MALDI-MS is promising to be a reliable high-throughput analytical method of small molecules.