植物体细胞融合育种能打破传统杂交育种和分子育种的局限，同时具有转入细胞质和细胞核中因而获得多个数量遗传性状等特点。而杂合体细胞再生植株是体细胞融合育种的瓶颈。项目组在前期研究中，建立和优化了新台糖22号和桂糖28号甘蔗体细胞融合育种体系并获得了再生植株，发现细胞再生能力与基因型和环境有密切关系，前人认为环境的对再生能力影响是与基因互作的结果。本项目拟利用 RNA-Seq技术研究甘蔗体细胞在酶解、钝化、融合、培养等阶段基因差异表达谱，明确与杂合体细胞再生密切相关的代谢途径，筛选关键候选基因，然后利用荧光定量 PCR 技术研究候选基因在杂合体细胞再生不同阶段表达模式及相关调控激素的关系，通过过表达和基因沉默技术验证候选基因的功能，建立杂合体细胞过程中相关基因的联结关系和调控网络，研究候选基因与细胞骨架的调控关系，为今后提高体细胞融合育种效率和精准调控甘蔗杂合体细胞再生植株奠定重要的理论基础。

Plant cell fusion breeding technology can overcome the limitations of traditional cross-breeding and modern molecular breeding by transferring large number of genes from the cytoplasm and the nucleus of target cell to newly produced cell, so that the new cell can possess and express multiple quantitative genetic traits from the cell of different cultivar. Regeneration is often the bottleneck in somatic hybridization breeding programs. In our previous studies, the fusion and breeding system of sugarcane somatic cell of ROC22 and GT28 had been established and optimized and the regenerated plants have obtained. Donor material genotype and environmental conditions have often been decisive for successful regeneration. The influence of environment on regeneration capacity is believed to be the result of interaction with genes. In view of this, the first, the different transcript expressing profile data of sugarcane cells in the stage of enzymatic hydrolysis, passivation, fusion and culture et al. were expressed by using RNA-seq techniques in this study, and the regulating network of regeneration occurring will be constructed, then the key genes response to regeneration plant occurring will be screened. Secondly the expressing character of the obtained key genes were evaluated in the process of regeneration occurring and treatment by using 6 regeneration regulating hormones, their functions will be verified by over expression and RNAi techniques. Finally, we will clone these key genes and analyze their expression in different relationship with hormone. Then we will know the molecular mechanism that how the mechanism of the regeneration sugarcane of hybrid somatic cells occurred. The knowledge obtained above contribute to regulate the regeneration plant occurring by using the key genes or pathways.