真眼点藻 (Eustigamatos sp.) 是一种可高效固定二氧化碳同化形成胞内储藏性三酰甘油 (TAG) 的产油单胞藻。本项目前期工作发现：氮胁迫条件下，该藻的光合色素减少、光合效率降低，而TAG却保持较快的积累速率，在此过程中藻细胞的光合代谢过程发生何种变化以满足TAG积累的能量需要？上述问题的研究对于阐明微藻产油机制具有重要意义。本项目从超微结构和光合效率角度，分析藻细胞光合能力与TAG积累的关系；利用叶绿素荧光探针，探究光合电子流分配；采用电泳、质谱与电镜技术，了解类囊体膜蛋白复合物的组成、排布及能量传递特性；结合转录组与荧光定量PCR，分析光合关键基因的转录表达；引入双向电泳和Western Blot技术，开展类囊体膜差异蛋白组学研究。本项目旨在揭示真眼点藻产油过程的光合响应及其分子调控机制，研究结果同时为“产油微藻高效光合固碳与TAG同步积累”的油脂高产策略提供理论依据。

Eustigamatos sp. is an oleaginous unicellular microalga which can accumulate large amounts of intracellular triacylglycerol (TAG) by photosynthetic carbon fixation. In nitrogen limitation, the photosynthetic pigments content decrease and the photosynthetic efficiency reduce, but TAG accumulation rate still keep a high level. Up to now, the internal mechanism of photosynthesis in response to lipid accumulation driven by nitrogen limitation supply is not yet entirely clear. Accordingly, the main research contents of this study include the following aspects: 1) the relationship between photosynthetic capability and triacylglycerol accumulation; 2) photosynthetic electron distribution in the process of lipid accumulation; 3) the subunits composition, assignment and photosynthetic energy transfer of pigment protein complexes; 4) the expression of the key photosynthetic genes at gene transcription level; 5) the differential proteome of thylakoid membrane based on two-dimensional electrophoresis and Western blot. The results will lead to deeply understand the correlation between photosynthesis and triacylglycerol biosynthesis in oleaginous microalgae. Moreover, the results probably give a theoretical guidance to enhancing the lipid productivity at high photosynthetic efficiency by regulating the concentration of nitrogen.