多疣壁虎断尾后能够进行皮肤、肌肉、软骨以及脊髓的再生，是一种理想的爬行类动物再生模型。断尾损伤后，愈伤上皮的快速覆盖和芽基形成是进行成功再生的关键事件，本研究以此为研究对象，在断尾再生（夏季断尾损伤）和非再生模型中（冬季断尾损伤）中探讨保守的Wnt和FGF信号通路对再生起始的作用。我们首先研究的是断尾再生起始过程中Wnt，FGF等通路在愈伤上皮及芽基组织中活化的时空特点；然后我们在断尾再生和非再生模型中探讨再生起始过程中Wnt和FGF的表达是否存在相互的调控作用以及对愈伤上皮及芽基组织的影响;进一步我们分析了非再生模型中低代谢水平以及活性氧分子对再生起始的效应以及对Wnt和FGF通路的影响。希望通过以上的研究为多疣壁虎断尾后再生模式的研究积累分子水平的数据，为理解不同物种器官和组织再生的机制增加新的内容。

Gekko japonicus, a species demonstrates multi-tissue regeneration capacity including skin, muscle, cartilage and spinal cord, is a feasible regeneration model for reptile. The quick cover of wound epithelium and the formation of blastema are the crucial events for the successful regeneration. We are going to investigate the role of Wnt and FGF pathway on the initiation of regeneration in the regeneration model (tail amputation in summer) and non-regeneration model (tail amputation in winter). First, we investigate the expression and activation pattern of Wnt and FGF pathway during the wound epithelium healing and blastema formation; Then, we explore the reciprocal role of Wnt and FGF activation and their effects on the epithelium and blastema cells. Finally, we investigate the mechanism of the epithelim failure in non-regeneration model which may associated with the low matabolism rate and ROS molecules. This study will provide the molecular data sets of regeneration, which might be useful to understand the molecular mechanism of the intrinsic capacity of organ or tissue regeneration in gecko and other species.