香蕉枯萎病是香蕉产业的毁灭性病害，其病原为尖孢镰刀菌古巴专化型（Fusarium oxysporum f.sp.cubense, Foc），其中Foc热带4号小种（Foc TR4）的危害最大。迄今为止，尚未发现有效的根治措施。抗病育种是最为有效和持久的选择，然而抗病品种的获得仍存在较大困难，根本原因在于高抗（甚至免疫）的香蕉资源和抗性基因的缺乏。本项目旨在对项目组收集的400余份香蕉种质资源进行抗病性评价，以获得优良的育种和实验材料；并以筛选获得的抗性最强和最弱的二倍体（AA）野生资源为材料，通过活细胞荧光成像和定量蛋白质组学等技术深入研究高抗资源对Foc TR4早期识别和免疫的生理学特征和分子机制，同时挖掘一些抗病基因。

Fusarium wilt of banana, the devastating disease of banana industry in the world, is caused by Fusarium oxysporum f. sp. Cubense (Foc). Foc tropical race 4 (Foc TR4) is one of the most destructive pathogen among all types of Foc. At present, there was no effective method to control the disease. Resistance breeding is the most effective and lasting strategy, but it is difficult to obtain resistant cultivars. The root cause is lack of high-resistant banana germplasm resources and genes. The project aims to evaluate the resistance of 400 banana resources, meanwhile excellent materials will be got for breeding and experiment. Then, using live-cell fluorescence imaging and Tandem Mass Tags (TMT) technology investigate the recognition and immune mechanism of the highest resistant and susceptive banana diploid (AA) to Foc TR4. At the same time, some resistant genes will be identified in the process.