Antisense RNA对基因表达调控具有重要作用。第二代链特异转录组测序技术促进了非编码RNA研究。申请人前期基于小鼠多组织细胞链特异性RNA-seq数据和表观修饰数据，进行了非编码RNA鉴定及表观修饰研究。本项目拟在前期研究基础上，增加小鼠肝和心脏等组织RNA-seq数据，整合公共数据，通过多组织和细胞RNA-seq数据分析，构建小鼠基因表达谱；鉴定Cis-和Trans-antisense RNA；研究Antisense RNA的特征及其与表观修饰的关系；揭示正义、反义链RNA表达相关性；分析Antisense RNA靶基因功能；构建小鼠多组织和细胞Antisense RNA数据库并提供在线Antisense RNA鉴定及分析。本研究对于注释小鼠非编码RNA(特别是Antisense RNA)、分析Antisense RNA特性、揭示正义、反义链RNA表达调控关系等具有重要意义。

The recent studies suggest that antisense transcripts play the important regulatory role in gene expression and the next generation sequencing technologies promote the study of non-coding RNA. In our lab, the applicant did non-coding RNA identification and epigenetic modification study based on the strand-specific RNA-seq data and epigenetic modification data for mouse. In this study, based on the preliminary studies, we will do RNA-seq for liver, heart and other important tissues, integrate public, old and new data, and do analysis for them as following: obtaining the gene expression profiles; identification cis- and trans-antisense RNA; study the characteristics of antisense RNA and the relationship between antisense RNA and epigenetic modifications; finding the association between sense and antisense RNA; analysis the function for target genes of antisense RNA; building antisense RNA database using these data, and providing antisense RNA identification and analysis for RNA-seq data based on web service. This project is very important and significant for annotation the non-coding RNA (especially for antisense RNA), anaylsis the characteristics of antisense RNA, and finding the regulating relationship between sense and antisense RNA in mouse.