Biotoxicity of Cry1Ab protein on wolf spider Pardosa pseudoannulata

Biotoxicity of Cry1Ab protein on wolf spider Pardosa pseudoannulata
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Cry1Ab蛋白对拟环纹豹蛛的生物毒性

DOI:
10.1007/s10646-017-1858-4
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发表时间:
2017-10
期刊:
影响因子:
2.7
通讯作者:
Song Qisheng
Song Qisheng
中科院分区:
环境科学与生态学4区
文献类型:
--
作者:
Wang Juan;Peng Yu;e;Yang Huilin;Yan Zhenzhen;Li Qinjin;Shi Yixue;Xie Chunliang;Liang Yunshan;Wang Zhi;Song Qisheng

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在这项研究中,我们开展了一项三营养生物测试,以评估表达Cry1Ab的水稻对常见狼蛛Pardosa puoannulata觅食行为的潜在影响及其潜在的分子机制。结果表明,与对照相比,含有Bt的蜘蛛表现出更大的觅食范围。进一步对摄食和不摄取Cry1Ab的拟环纹夜蛾中枢神经系统进行了高通量新转录组测序。共获得120.985个非基因片段,平均长度为529.73个碱基。差异表达基因的功能分析表明,与能量代谢相关的基因的表达水平随着Cry1Ab的变化而发生变化,这可能有助于更积极的觅食行为。此外,一些DEGS还具有与金属离子结合相关的功能,这意味着可能对金属离子依赖的反应产生影响。这可能与蜘蛛对Cry1Ab的抗性机制有关。
In this research, we carried out a tritrophic bioassay to assess the potential effect of Cry1Ab-expressing rice on the foraging behavior of the common wolf spider Pardosa pseudoannulata and its underlying molecular mechanism. Results indicated the Bt-containing spiders expressed a higher foraging range when compared to controls. The high throughput de novo transcriptome sequencing was further carried out for central nervous system (CNS) of P. pseudoannulata with and without Cry1Ab intake. We obtained 120, 985 unigenes with a mean length of 529.73 bp. Functional analysis of differentially expressed genes (DEGs) showed the expression levels of genes related to energy metabolism were changed in response to Cry1Ab, which may contribute to a more active foraging behavior. In addition, some DEGs also have a function related to metal ion binding, implying a potential influence on metal ions-dependent reactions. This may be associated with Cry1Ab resistance mechanism in the spider.
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发表时间: 2008-06
影响因子: 6.8
作者:
R. Ramírez-Romero;Nicolas Desneux;A. Decourtye;Antoine Chaffiol;M. Pham-Delègue
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