生物内源性活性物质（Biological endogenous active substance, BEAS）是一类具有生理功能和生物学活性的生物分子，与生命系统中众多生理和病理过程息息相关。发展准确、灵敏的BEAS即时检测方法对于生命基础研究及疾病的预防、早期诊断和预后判定等均具有重要的科学意义。本项目拟发展一种基于酶@金属有机框架（酶@MOFs）和纳米金棒体系的准确、灵敏BEAS即时分析平台。项目将合成系列糖类等BEAS快速、稳定、灵敏响应的酶@MOFs级联纳米反应器，并制备以酶@MOFs为识别单元，纳米金棒为信号输出单元的可视化试纸型传感装置；同时，研发可直接处理颜色信息的智能手机App，建立基于智能手机-试纸传感装置联用的准确、灵敏便携式即时分析平台。本项目的开展不仅可为BEAS的快速、准确检测提供新分析方法，也为稳定、灵敏、经济的便携式即时分析系统提供新思路。

Biological endogenous active substance（BEAS）is a class of biological molecules with physiological functions and biological activities, which are known to be involved in a wide range of biological and pathological processes. The development of rapid and accurate BEAS detection methods is of great scientific significance for basic life research, disease prevention, early diagnosis and prognosis. This project intends to develop an accurate and sensitive portable BEAS real-time analysis platform based on enzymes@metal organic frameworks (enzymes@MOFs) and gold nanorod system. Firstly, it will develop a series of rapid, stable and sensitive saccharides-responsive enzymes@MOFs cascaded nanoreactor, and then prepare a visual paper-based sensing device with enzyme@MOFs as the recognition unit and gold nanorod as the signal output unit. Meanwhile, it will also develop a smartphone app that enables to directly process the color information, and finally establish the portable intelligent real-time analysis platform based on the paper-based sensing device coupled with the smartphone. This project can not only provide a new analytical method for the rapid and accurate detection of BEAS, but also advance the idea for the development of stable, sensitive and economical portable real-time analysis system.