牛脂肪细胞增殖分化过程中关键候选lncRNA BADLNRs 的功能及其调控机制研究

脂肪细胞增殖分化是影响肉牛生长发育与肉质的重要因素之一，其机理研究有利于解决我国当前地方黄牛品种生长慢、产肉率低等问题。长链非编码RNA(lncRNAs)在物种间保守性低，对牛脂肪细胞增殖分化具有重要调控作用，但存在哪些关键lncRNAs及其功能、调控机制如何，尚不清楚。因此，本项目以秦川牛为对象，在已有前体脂肪细胞分化过程中差异表达lncRNA数据的基础上，鉴定出4个新lncRNA BADLNRs（即BADLNR1～4)，利用功能获得与缺失策略研究它们对脂肪细胞增殖分化的调控作用，探索lncRNA-miRNA的共调控机理；此外，借助RIP、ChIRP等技术研究与BADLNRs相互作用的蛋白和染色质，阐明关键lncRNA-miRNA-靶蛋白对牛脂肪细胞增殖分化的具体调控机制。该项目旨在阐明关键候选lncRNAs在脂肪增殖分化过程中的功能与调控机制，为肉牛快速生长与肉质改良奠定基础。

Proliferation and differentiation of adipocyte is one of the most important factors for growth and development and meat quality in beef cattle. Its mechanism understanding will help us solve the problems of low growth rate and meat production in Chinese yellow cattle. Long non-coding RNAs (lncRNAs), which are not very conserved across species, play a significant role during the cattle preadipocyte proliferation and differentiation. However, we do not know the detailed crucial lncRNAs in adipocyte proliferation and differentiation, as well as their function and regulation mechanism are still unknown. Base on above facts, we intend to combine the existing data we have obtained from screening differentially expressed lncRNAs in progress of preadipocyte maturation, and we will identify four novel lncRNA BADLNRs (bovine adipocyte differentiation lncRNAs), namely, BADLNR1～4. Using overexpression and silence strategy, the function and regulation mechanisms of these candidate lncRNAs will be studied in adipocyte proliferation and differentiation, as well as their co-regulation mechanism with lncRNA-miRNA will be analyzed. In addition, the RIP and ChIRP techniques will be used to detect those proteins and chromatins interacting with BADLNRs, to elucidate the regulation mechanisms of lncRNA-miRNA-target protein process controlling the cattle adipocyte proliferation and differentiation. Briefly, this study aims to clarify functions and regulatory mechanisms of the key candidate lncRNAs in the preadipocyte proliferation and differentiation. These results will provide a solid theoretical foundation to improve beef cattle growth rate and meat quality.