细胞外囊泡（EVs）及异质性亚群外泌体（EXOs）和微囊泡（MVs）作为细胞间交流和信号转导的载体为颗粒物（PM）致心血管损伤(CVD)研究提供了新思路。本研究针对EVs提取、纯化及EXOs和MVs分离分析中的难点问题，拟利用TIM4与磷脂酰丝氨酸亲和反应，简便、快速、高效提取EVs；应用新兴的非对称流场流分离技术（AF4）偶联多检测器（UV、MALS、DLS），结合表面增强拉曼（SERS）技术，建立分离条件温和、分辨率高且分析范围宽的EXOs和MVs快速分离检测体系。基于AF4/UV-MALS-DLS-SERS研究超细颗粒物（PM0.1）对血小板来源的EXOs和MVs的影响；通过细胞实验和动物实验明确PM0.1介导EVs对心血管系统的损伤作用。本研究将为PM致CVD的研究提供理论基础，为应用AF4深入探讨EVs的生物学效应，促进EVs在致病机理、疾病诊断与治疗中的应用提供关键技术支撑。

As carriers of intercellular communication and signal transduction, extracellular vesicles （EVs）and its subpopulations exosomes (EXOs) and microvessels (MVs) provide a new idea for the study of cardiovascular damage (CVD) induced by particle matter (PM). In view of the difficulties in the extraction and purification of EVs and the separation of EXOs and MVs, we extract high purity EVs using the affinity reaction of TIM4 and phosphatidylserine. Then, we establish the detection system of EXOs and MVs with a mild separation conditions,high resolution and wide range analysis using a novel asymmetric flow field separation (AF4) coupled with multiple detectors (UV, MALS, DLS) and surface enhanced Raman scattering (SERS). The effects of ultrafine particles (PM0.1) on platelet-derived EXOs and MVs is investigated based on AF4 / UV-MALS-DLS-SERS. Through cell experiments and animal experiments, the damage effects of PM0.1-mediated EVs to cardiovascular system is identified. This study will provide the theoretical basis for the research of PM induced CVD, and provide the key technical support for the further study of the biological effects of EVs to promote the application in the pathogenesis, disease diagnosis and treatment.