纳米颗粒物暴露是当今最紧迫的公共卫生问题，与人类心血管疾病的发生发展密切相关。凝血与抗凝血平衡失调可以导致多种心血管疾病发生，研究纳米颗粒物与凝血相关因子相互作用的分子机理是深入探讨其凝血机制的基础。本课题利用新兴的非对称流场流分离技术（AF4）偶联紫外检测器（UV）、多角度激光散射仪（MALS）和电感耦合等离子体质谱仪（ICP/MS），建立样品处理简单、分离条件温和、分辨率高且分析范围宽的AF4-UV-MALS-ICP/MS分离检测体系，研究纳米颗粒物与凝血相关因子的结构形态变化，相互作用的热力学特征（作用力，键合点，亲和性）和结合动力学特征（吸附率，解离速率），明确纳米颗粒物与凝血相关因子作用的分子机理。结合动物实验，研究纳米颗粒物对机体凝血功能的影响。本研究将为纳米颗粒物致心血管疾病的深入研究提供理论基础，为应用AF4分离系统研究纳米颗粒物与生物大分子相互作用提供关键技术支撑。

Nanoparticals exposure is one of the most pressing public health issues, and is closely related to the occurrence of human cardiovascular disease. The imbalance of coagulation and anti-coagulation can lead to a variety of cardiovascular disease. Study on the interaction and molecular mechanism of nanoparticals and coagulation-related factors is the basis for an in-depth exploration of the coagulation mechanism. This study is to establish the detection system via asymmetric flow field -flow fractionation (AF4) coupled with ultraviolet-visible (UV) detector, multi - angle laser light scattering (MALS) detector and inductively coupled plasma mass spectrometer (ICP/MS), and study on the configuration and structure, thermodynamic characteristics (force, bonding, affinity), dynamics characteristics (adsorption percentage, dissociation rate) and understand the molecular mechanism of nanoparticles and coagulation-related factors interaction. Combined with animal experiment, study on the influence of nanoparticles on the organism coagulation function. The study will provide a theoretical basis for further research of cardiovascular disease caused by nanoparticles and providing key technical support for the application of AF4 separate system to study nanoparticles- biological molecules interaction.