草莓匍匐茎发生调控基因的定位与鉴定

匍匐茎繁殖是草莓繁殖的主要方式，有关草莓匍匐茎发生的机理，至今主要集中在生理层面，而分子层面的机理尚不清楚。我们从不抽生匍匐茎的二倍体森林草莓（Fragaria vesca）中人工诱变出抽生匍匐茎的突变体，遗传分离比例显示该突变性状由隐性单基因（srp）控制。基于杂交亲本基因组重测序数据，我们开发出了SSR和InDel标记，并初步将srp基因定位于4号染色体2.5cM范围内。基于上述研究基础，本项目拟构建隐性个体数大于1000的F2群体，采用分子标记（SSR、InDel）与全基因组重测序BSA相结合的方法进行srp基因的定位，同时结合转录组数据，筛选调控草莓匍匐茎发生的关键候选基因。克隆候选基因并构建其过量表达载体和RNAi载体，通过草莓转基因来鉴定候选基因功能。对候选基因表达产物进行亚细胞定位并分析其表达特性。本项目旨在克隆调控草莓匍匐茎发生基因，为揭示草莓匍匐茎发生分子机理奠定基础。

The propagation of strawberry is mainly based on runners. Mechanism of produce of strawberry runners is majorly focused on physiological aspect rather than molecular level. We have obtained a mutant with runners from woodland strawberry (Fragaria vesca) which do not have runners through chemomorphosis. Genetic analysis revealed that the phenotype of produce of strawberry runners was controlled by a single recessive gene (srp). SSR and InDel markers were developed according to the re-sequencing of parental genome and srp gene was initially delimited to 2.5cM genetic distance on chromosome 4. Based on these data, we will construct a large F2 population with more than 1000 recessive plants with mutant phenotype. SSR and InDel markers and whole genome re-sequencing BSA methods will be used to perform fine-mapping of the candidate gene. In addition, we will combine transcriptome data with gene mapping data to obtain the key candidate genes regulating the produce of strawberry runners. Candidate gene clone, overexpression and RNAi vectors will be conducted to investigate the function of candidate genes. Subcellular localization and spatial and temporal expression pattern will also be analyzed in this project. The aim of this project is to clone the gene regulating the produce of strawberry runners and lay a solid foundation for uncovering the molecular mechanism of the produce of strawberry runners.