基于功能化微纳流控芯片系统的生物分子识别研究

本项目拟以生物分子识别研究为基点，结合纳流控的高效富集能力与适配体探针的高选择性优点，创建基于功能化微纳流控芯片系统的生物分子识别新方法。根据拟定的研究目标，本项目将纳米结构单元引入微流控系统，构建功能化微纳流控芯片系统，以适配体与蛋白质相互作用为研究对象，利用微纳流控系统独特的物质传输性质，分别构建异相和均相生物分子识别反应体系，实现连续、微型化方式对蛋白质样品进行快速富集、识别、分离与检测，揭示不同反应体系下蛋白质识别反应动力学，并系统研究探针的组装方式、通道尺寸、表面性质、外加电场强度、样品富集时间、溶液组成等因素对蛋白质识别的影响，创建基于功能化微纳流控芯片系统的高灵敏生物分析新方法。研究成果将对认知生命体系中的生物分子识别过程提供理论依据，同时为微纳流控芯片系统在生物分析中的应用起到重要的推动作用。

This project will focuse on the study of interactions between biomolecules. To realize this aim, a novel concept based on functional micro/nanofluidic system will be developed, which combines the excellent preconcentration ability of nanofluidics and the high selectivity of aptamer. In this sutdy, the functional micro/nanofluidic system for protein recognition is first constructed by introducing nanostructures. Then, homegeneous and heterogeneous biomoelcuar recognition systems will be designed to realize various functions including rapid protein concentration, molecular recognition, efficient separation, and sensitive detection on micro/nanofluidic chips in miniaturized and continuous manner and understand the protein recognition kinetics in heterogeneous and homogeneous reaction systems. Finally, the influenece of probes immobilization, channels size, surface properties, electric field intensity, sample concentration time, and solution constitution on the protein recognition reactions will be investigated systematically. On the basis of the above researches, a novel bioanalytical systems based on micro/nanofluidics with high sensitivity is constructed. We believe that progresses achieved in this project will help us to understand the fundamentals of biomoelcular recognition reactions and promote the developement of micro/nanofludic systems.