WRKY72调控水稻种子休眠的机制研究

脱落酸(ABA)是调控水稻种子休眠的重要激素之一。前期研究结果显示转录因子WRKY72受ABA显著诱导，在种子里具有较高的表达量，其超表达材料的种子萌发与野生型相比对ABA的敏感性增加，表明WRKY72可能参与ABA调控的种子休眠过程。组织定位和各种逆境响应表达模式分析暗示WRKY72可能作为一个正调控因子参与ABA信号途径；通过发展 WRKY72超表达、wrky72-crispr突变体以及突变体功能回复材料，比较种子萌发及幼苗生长对ABA的敏感性及该过程中的生理测定在转基因植株与野生型间的差异，探究WRKY72在调控种子休眠过程中的生物学功能。另一方面，本项目还分别对WRKY72超表达株系受ABA处理和不受ABA处理的萌发后种子进行转录组学分析，筛选出受WRKY72直接调控的ABA信号通路的下游靶基因，发现WRKY72参与ABA调控种子休眠的新信号途径，为分子育种提供一定的理论基础。

As one of the most important phytohormones，abscisic acid (ABA) regulated the seed dormancy in plants. Our previous studies have shown that transcription factor WRKY72 was significantly induced by ABA and highly expressed in seed. Moreover, the seeds of WRKY72 over-expression transgenic rice emerged more sensitive to ABA stress compared with wild-type, that was seed dormancy became deepened and seed germination was retarded under ABA treatment, suggesting WRKY72 may be involved in ABA-mediated seed dormancy and germination processes. This project aims to clarify the biological function of WRKY72 in rice seed dormancy, and to explore the new molecular ABA-mediated seed dormancy signaling pathway in which WRKY72 participates. Analysis of tissue location and expression patterns response to various stresses indicated that WRKY72 may act as a positive regulator involved in ABA signal pathway. Through generating WRKY72 over-expression, wrky72-crispr mutant and function recovery transgenic lines, the sensitive of seed germination and seedling growth to ABA stress as well as physiological determination will be compared among the transgenic plants and wild-type, thus to interpret the biological function of WRKY72 in seed dormancy. On the other hand, comparative transcriptomic analysis of germinated seeds of WRKY72 over-expression lines under ABA and no ABA treatment will be performed, and the target genes involved in ABA signal pathway will be screened and detected whether direct regulated by WRKY72. Given the importance of abscisic acid signal network in rice seed dormancy and germination processes, the outcome of this project will not only expand the molecular mechanism of ABA-mediated seed dormancy, but also provide a theoretical basis for molecular breeding.