我国青少年近视患病率全球最高,选择准分子激光原位角膜磨镶术(LASIK)矫正视力人数急剧攀升。由于待矫正度数正比于切削厚度,所以临床上常采用保守切削厚度以降低可对视力造成破坏性影响的术后继发性角膜膨隆的发生。由于缺乏对术后自修复与老化过程中角膜生物力学特性的深刻认识,切削厚度设计个性化不到位,致使术后继发性角膜膨隆等问题凸现。本研究拟对四组兔实施切削厚度不同的LASIK术,其中两组切削厚度大于临床保守厚度。在2年间定期在体采集角膜形态学和眼压等信息；定期对各组实施离体角膜的力学测试与微观结构观察；确定描述角膜各向异性粘弹性的材料参数；采用有限元计算进行验证并模拟眼反应分析仪(ORA)的测试过程；揭示不同切削厚度的术后角膜在自修复及老化过程中的生物力学规律，探索 ORA模拟结果与材料参数之间的联系,期望给出安全切削厚度的临界条件。本项目的研究结果可为屈光手术的个性化设计与术后评估提供理论基础

China has the highest rate of youths affected by myopia, leading to a surge in number of patients taking Laser-Assisted in situ Keratomileusis (LASIK). Due to a lack of understanding of corneal mechanical properties in the post-operative self-repair and ageing process, cutting depth is not personalized, giving rise to post-operative secondary corneal ecstasia. Our study will carry out LASIK on four groups of rabbits with different cutting depth, two of which have deeper cuts than clinical conservative ones. We will collect corneal morphology and eye pressure data, carry out in vitro corneal mechanical tests and observation of microscopic structures on a regular basis; use finite-element method to demonstrate and simulate measurement from ocular response analyzer (ORA). We will study the change of corneal mechanical properties during its self-recovery and ageing process, the relationship between ORA simulation results and material parameters, to give the critical cutting depth. This study could lay the foundation for personalized design of LASIK, and a theoretical basis for postoperative evaluation.