锌指蛋白（ZFP）参与桃果实采后抗冷性调控机制研究

冷害是桃果实在采后冷藏过程中存在的主要问题之一。前人主要从细胞结构与生理代谢学角度研究桃果实冷害发生机制。近年来，转录因子介导的转录调控在果实采后抗逆性中的作用日益被人们所重视。锌指蛋白是一类广泛存在于真核生物中的转录因子，在植物响应逆境胁迫和冷信号转导过程中具有重要的生物学功能。然而，锌指蛋白与桃果实采后抗冷性的关系，以及调控冷害的分子机制尚不明确。我们前期研发发现，桃果实采后抗冷诱导提高伴随着多个锌指蛋白基因的显著上调。因此本项目以桃果实为试材，以锌指蛋白对冷信号的响应和转导作为科学问题切入点，深入研究桃果实冷害发生过程中锌指蛋白下游调控的靶基因和特异性因子、互作蛋白和抗冷蛋白泛素化修饰的差异表达。旨在阐明锌指蛋白在桃果实抗冷性调控中的生物学功能。研究结果有望从转录调控角度部分揭示桃果实抗冷性调控中的分子机制，为进一步阐明果实采后冷害的调控机理提供新的理论依据。

Chilling injury (CI) is a major problem in the peach refrigeration, which reduces the quality and limits the storage period. Although, previous studies reported various postharvest treatments reduced CI in peach fruit, which focused on the mechanism involved in cell structure and physiological metabolism. In recent years, it has been paid more and more attention in the role of regulation mechanism by transcription factors in postharvest stress resistance. Zinc finger proteins (ZFP), a kind of transcription factor widely existing in eukaryotes, play an important role in plant response to stress and cold signal transduction. However, the relationship between ZFP and chilling resistance of peach fruit and their molecular mechanism of regulating CI is still not clear. In our previous study, in was found that cold resistance was enhanced in peach fruit by postharvest treatment accompanied by up-regulation of various ZFP genes. Thus, this study focused on the scientific issue of ZFP responding to cold stress in peach fruit, and commenced on the expression of downstream target genes and specific factors, interacting proteins and ubiquitination of cold regulated proteins associated with CI. This study is supposed to illuminate the regulatory mechanism and biological function of ZFP responding to cold stress in peach fruit. Moreover, the results of this study are expected to partially reveal the molecular mechanism of cold resistance in peach fruit involved in transcriptional regulation, and provide new theoretical principle in controlling CI and improving quality in peach fruit during cold storage.