基于钙调素响应冷信号的桃果实采后抗冷害机制研究

冷害是桃等冷敏性果实采后贮藏发生的主要生理性病害，限制了桃果实贮藏寿命和冷藏技术的应用，研究桃果实采后冷害发生机制和调控措施具有重要的理论意义和应用价值。钙调素作为钙离子受体蛋白参与植物对低温等逆境胁迫的分子调控，钙调素在响应冷信号、下游信号通路转导和调节蛋白磷酸化等方面都具有重要功能。本项目以冷敏性桃果实试材，以钙调素对冷信号的响应和转导作为科学问题切入点，深入探究桃果实冷害发生过程中钙调素结合蛋白、钙调素转录因子、钙/钙调素依赖蛋白磷酸化和下游冷信号转录的差异表达，阐明钙调素在桃果实冷信号转导中的分子通路和代谢网络，揭示钙调素在桃果实响应冷信号的关键枢纽作用。本项目的研究成果可丰富果实采后冷害调控的生物学理论，为控制桃果实采后冷害劣变提供理论依据。

Chilling injury (CI) is a major physiological disorder in peach fruit during cold storage, which limits the storage period of peach fruit and the application of refrigeration. It is important to evaluate the mechanism and controlling method of CI both in theoretical basis and practical implications. Calmodulin (CaM), as calcium receptor proteins, participates the molecular regulation against cold stress and plays important roles in response to cold signal, transduction of downstream signal pathways and regulation of protein phosphorylation in plant. This study focused on the scientific issue of CaM responding to cold stress in cold-stored peach fruit, and commenced on the expression of CaM and CaM-binding transcription activator gene, proteomics analysis of Ca2+/CaM dependent protein phosphorylation and regulation of downstream transcription factor associated with cold signal pathways. Thus, this study is supposed to illuminate the signal pathways and metabolic network of CaM regulating the transduction of cold signal, and explain the key role as a bridge of CaM responding to cold stress in cold-stored peach fruit. Moreover, the results of this study can enrich the theory of CI regulation in postharvest biology, and provide theoretical principle in controlling CI and deterioration in peach fruit during cold storage.