插穗的“不定”生根及其应答逆境胁迫的可塑性发育，既是林木无性系选育与推广的关键，也关乎林木在复杂土壤环境中的资源获取方式。本项目拟从不定根原基形成的细胞学观察着手，研究特定组织或细胞对创伤、逆境胁迫等信号的发根感受性，挖掘和鉴定该过程中时空特异表达的长链非编码RNA（lncRNA）。经全长克隆、分子特征分析及蛋白编码能力验证，构建候选lncRNA的过表达和CRISPR/Cas9敲除载体，开展杨树遗传转化；结合微扦插生根试验和不同逆境胁迫处理，探究lncRNA在杨树不定根可塑性发育中的生物学功能。同时，进一步采用多种技术方法在转录、转录后以及表观遗传水平解析重要lncRNA与功能基因、miRNA以及蛋白质的互作关系，从而揭示它们在杨树不定根可塑性发育过程中的作用机制。该研究不仅有助于丰富和拓展植物器官发生与形态建成的理论基础，也为增强林木逆境适应性及改良扦插生根性状提供重要的科学依据。

The adventitious rooting of cuttings and their plastic development under various adversity stress are not only the key to clonal breeding and promotion forestry, but also the resources acquisition way of trees in complex soil environments. Starting with the cytological observation of the formation of adventitious root primordia, this project will investigate their rooting sensitivity to signals of wound and stress, and identify developmental-specific long non-coding RNAs (lncRNA) in this process. Through full-length cloning, molecular characterization analysis, verification of protein coding ability, candidate lncRNA overexpression and CRISPR/Cas9 knockout vectors will be constructed to carry out poplar genetic transformation. The biological functions of candidate lncRNAs in adventitious root plasticity development would be explored by combining with various stress treatments in micro-cutting rooting test. Meanwhile, the interaction of important lncRNAs with functional genes, miRNAs and proteins will be further analyzed at the transcriptional, post-transcriptional and epigenetic levels, to reveal the mechanism of their roles in the development of adventitious root plasticity in poplar. This study will not only enrich and expand the theoretical basis of plant organogenesis and morphogenesis, but also provide an important scientific basis for enhancing the adaptability of trees to adversity and improving the rooting character of cuttings.