Molecular Cloning, Transcriptional Profiling, Subcellular Localization, and miRNA-Binding Site Analysis of Six SCL9 Genes in Poplar.
Molecular Cloning, Transcriptional Profiling, Subcellular Localization, and miRNA-Binding Site Analysis of Six SCL9 Genes in Poplar.
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DOI：
10.3390/plants10071338
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发表时间：
2021-06-30
期刊：
影响因子：
--
通讯作者：
Xu M
中科院分区：
文献类型：
--
作者：
Zhao M;Xuan L;Qi H;Shen T;Xu M
SCL 9亚家族是GRAS家族的关键成员，调节植物发育和胁迫反应。然而，这些基因在白杨生长发育中的功能作用尚不清楚。在此，我们报道了6个在白杨不定根形成过程中差异表达的SCL 9基因。利用RACE技术克隆了“南林895”白杨（Populus deltoids × Populus euramericana）的PeSCL 9基因的全长序列。RT-qPCR结果显示，PeSCL 9基因在白杨不定根中呈动态表达。利用生物信息学方法对6个基因进行了一系列综合基因特征分析。同时，对杨树原生质体的瞬时表达分析表明，所有的PeSCL 9蛋白都定位于细胞核中。此外，结合南林895白杨的degradome和sRNA，对调控PeSCL 9的miRNAs进行了预测。结果表明，miR 396 a和miR 396 c可能通过切割作用影响PeSCL 9的表达，并通过杨树原生质体瞬时表达实验进一步验证了这一点。这6个基因与白杨不定根及其他组织的发育密切相关，是进一步研究白杨生长发育调控机制的起点。
The SCL9 subfamily is a key member of the GRAS family that regulates plant development and stress responses. Nevertheless, the functional role of these genes in the growth and development of poplar still unclear. Here, we reported the six SCL9 genes, which were found to be differentially expressed during poplar adventitious root formation. The full-length sequences of PeSCL9 genes of ‘Nanlin895’ poplar (Populus deltoids × Populus euramericana) were cloned by the RACE technique All PeSCL9 genes lacked introns. RT-qPCR revealed that PeSCL9 genes displayed a dynamic expression pattern in the adventitious root of poplar, according to RT-qPCR data. A series of comprehensive genes characteristics analysis were carried out for six genes by bioinformation. Meanwhile, transient expression analysis of the Populus protoplasts showed that all the PeSCL9 proteins were localized in the nucleus. In addition, the degradome and sRNA of ‘Nanlin895’ poplar in combination were used to predict miRNAs that regulate PeSCL9. It was found that miR396a and miR396c may affect PeSCL9 expression via cleavage, which was further verified by a transient expression experiment in Populus protoplasts. Overall, the development of poplar adventitious root and other tissues was closely related to these six SCL9 genes, and they serve as a starting point for further research into the mechanisms regulating poplar growth and development.
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