已有研究证实棕色脂肪组织可改善多囊卵巢综合征（Polycystic Ovary Syndrome, PCOS）代谢-生殖紊乱表型，因此，阐明棕色脂肪发育及其上下游信号，可揭示棕色脂肪对PCOS复杂代谢-生殖病生理改变的调节机制。本课题在前期成功构建了Kit-CreER；Rosa26-RFP谱系示踪小鼠模型基础上，将首次利用谱系示踪技术阐明c-Kit+干/祖细胞在棕色脂肪发育中分化命运，单细胞测序技术进行棕色脂肪细胞图谱聚类分析，并观察c-Kit+来源棕色脂肪细胞对代谢生殖调节作用，结合代谢组学研究 PCOS 模型中代谢重塑对 c-Kit+ 干/祖细胞向棕色脂肪组织分化的调节作用及内在机制，拟揭示高雄激素水平影响棕色脂肪发育与PCOS代谢生殖紊乱发生相关性及其调控机制，对PCOS的防治提供新思路。

It has been reported that brown adipose tissue (BAT) could improve dysfunction of metabolism and reproduction in polycystic ovary syndrome (PCOS). Therefore, to elucidate the underlying mechanism of BAT development, may help to reveal how BAT play a role in complex PCOS pathophysiology. Previously, we have already set up a c-Kit+ lineage tracing model, Kit-CreER；Rosa26-RFP mice. In this study, we will observe c-Kit+ stem/ progenitor cell fate in brown adipose tissue development by using Kit-CreER；Rosa26-RFP mice. Single cell sequencing will be employed to analyze cell components of brown tissue. Metabolomics mechanism also will be investigated to elucidate that metabolism remodeling in PCOS mouse brown adipose tissue may regulate pathways involving in c-Kit+ stem/ progenitor cell differentiation. The study of BAT development and its underlying mechanism involved with PCOS pathophysiology will provide a new strategy to protect and treat PCOS.