Autoimmune thyroid disease disrupts immune homeostasis in the endometrium of unexplained infertility women-a single-cell RNA transcriptome study during the implantation window.
Autoimmune thyroid disease disrupts immune homeostasis in the endometrium of unexplained infertility women-a single-cell RNA transcriptome study during the implantation window.
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DOI：
10.3389/fendo.2023.1185147
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
已知自身免疫性甲状腺疾病（AITD）与女性不明原因不孕有关。虽然抗甲状腺抗体的存在被推测是免疫失衡的标志，可能导致植入失败，但其影响子宫内膜容受性的潜在机制仍有待阐明。本研究采用单细胞RNA测序（scRNA-seq）技术对AITD患者着床窗期（WOI）子宫内膜免疫微环境进行解剖。我们收集了不明原因不孕症合并AITD妇女（n=3）和AITD-对照（n=3）子宫内膜样本的CD45+免疫细胞群。然后用10X Genomics Chromium处理细胞进行进一步分析。我们总共鉴定了28种不同的免疫细胞亚型，并揭示了AITD患者和对照组之间的组成和基因表达模式的差异。AITD+患者T CD4+、cNK、ILC3、T CD8+ GZMK+、T CD8+细胞毒性和ILC3 CD3E -细胞比例升高，CD366+ uNK1降低。AITD患者中GNLY及趋化因子表达异常。此外，uNK和T CD8+细胞毒细胞表现出较低的细胞毒性，但激活了免疫反应。ILC3和Tregs细胞粘附富集基因下调，ILC3和Tregs数量增加。WOI期间子宫内膜存在免疫失衡，可能影响胚胎着床。
Autoimmune thyroid disease (AITD) is known to be associated with unexplained infertility in women. Although the presence of antithyroid antibodies have been speculated to be a marker of an immune imbalance that might lead to implantation failure, its underlying mechanism influencing the endometrial receptivity remains to be elucidated. In this study, we used single-cell RNA sequencing (scRNA-seq) to dissect immune microenvironment in endometrium of AITD patients during window of implantation (WOI). We collected CD45+ immune cell populations of endometrium samples of unexplained infertile women with AITD (n=3), as well as samples of AITD- controls (n=3). The cells were then processed with 10X Genomics Chromium for further analysis. We characterized 28 distinct immune cell subtypes totally, and uncovered differences in the composition and gene expression patterns between AITD patients and controls. The proportions of T CD4+, cNK, ILC3, T CD8+ GZMK+ , T CD8+ Cytotoxic and ILC3 CD3E - cells were increased, and CD366+ uNK1 was decreased in AITD+ patients. And the abnormal expression of GNLY and chemokines was observed in AITD patients. In addition, uNK and T CD8+ Cytotoxic cells showed lower cytotoxicity but activation of immune response. Genes enriched in cell adhesion of ILC3 and Tregs were downregulated, while the number of ILC3 and Tregs were increased. Immune imbalance exists in endometrium during WOI, which may impact embryo implantation.
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影响因子：
7.3
作者：
Gaynor LM;Colucci F
通讯作者：
Colucci F
影响因子：
4.4
作者：
Einenkel, Rebekka;Ehrhardt, Jens;Zygmunt, Marek;Muzzio, Damian Oscar
通讯作者：
Muzzio, Damian Oscar
DOI：
10.1111/j.1600-0897.2009.00792.x
发表时间：
2010-01
期刊：
American journal of reproductive immunology (New York, N.Y. : 1989)
影响因子：
--
作者：
Dekel N;Gnainsky Y;Granot I;Mor G
通讯作者：
Mor G
DOI：
10.1080/14767058.2020.1740675
发表时间：
2020-03-18
影响因子：
1.8
作者：
Du, Mingyue;Wang, Weijia;Li, Liping
通讯作者：
Li, Liping
影响因子：
5.6
作者：
Azizi, Ramyar;Ahmadi, Majid;Yousefi, Mehdi
通讯作者：
Yousefi, Mehdi