本项目针对糖尿病血管病变广泛且无可用的替代血管现状，基于干细胞能量代谢稳态维持及单核细胞表型转化在构建抗高糖损伤的工程血管中的重要作用。在本课题组前期发现腺苷促进内皮祖细胞归巢于工程血管显著提高工程血管通畅率重要进展基础上，发挥课题组在工程血管体内外构建中的优势，进一步揭示调控腺苷激酶在干细胞能量代谢稳态维持及促进单核细胞表型转化中的作用及机制，进而构建抗高糖损伤的工程血管：1）腺苷激酶调控对内皮祖细胞能量代谢稳态维持进而抗高糖损伤的作用及机制；2）腺苷激酶促进内源性腺苷释放到胞外，调控单核细胞表型转换的作用及其机制；3）制备光交联抗凝材料，将腺苷激酶siRNA纳米缓释颗粒和CD34特异性抗体复合应用于工程血管表面修饰，构建抗高糖损伤及内膜增生的工程血管。项目的完成将为构建抗疾病损伤的血管植入物提供新的方法。

This project is aimed at the extensive vascular disease of diabetes and no available alternative TEVB , based on cellular energy homeostasis of stem cell and monocytes conversion plays an important role for constructing a TEBV. We previously found that adenosine can promote endothelial progenitor cells homing to the engineering vessel and can significantly increase the patency rate of TEBV ,based on the advantage in constructing TEBV in vivo and in vitro, further revealing the mechanism of adenosine kinase for the homeostasis of endothelial progenitor cell energy metabolism and monocytes conversion in diabetes mellitus, and constructing a TEBV of resisting to high glucose injury. 1)researching the characteristics and mechanism for the homeostasis of endothelial progenitor cell energy metabolism of adenosine kinase in high glucose environment; 2)When endogenous adenosine released into the extra cellular, researching on the effects and the mechanism of regulation of monocytes conversion; 3) Preparing of photocrosslinked anticoagulant materials, the adenosine kinase siRNA slow releasing nano particles and the specific antibody of CD34 composite are applied to surface engineering vascular modifications, researching on the construction of engineered blood vessel which can resist high glucose injury and intimal hyperplasia. The completion of the project will provide a new method for constructing blood vessel implant anti -disease injury.