龋损修复是口腔临床最常见的重要工作，目前存在边缘微漏、牙髓刺激性、材料老化等问题。借助仿生矿化思想，在龋损原位再生牙体硬组织可能成为龋损修复的理想新模式。迄今国内外尚缺乏这方面的系统研究，仿生矿化修复龋损牙体组织的应用仍是空白。基于釉原蛋白、牙本质磷蛋白在牙发育过程中的生物矿化调控作用及体外其他高分子诱导羟磷灰石合成的成功，申请人创新提出仿生矿化原位修复牙体龋损思想，对课题组前期获得的具有再矿化作用的釉原蛋白功能多肽和牙本质磷蛋白功能多肽8DSS进行结构优化，通过多体系仿生矿化活性筛选实验，构建牙体硬组织仿生矿化功能多肽，通过多肽作用界面、钙磷供给形式等的研究建立仿生体外模型，评估其原位修复龋损有效性，探索其作用机制。本研究为仿生矿化功能多肽修复牙体龋损的应用提供理论依据，为开辟一条新的安全有效的牙体龋损修复方法奠定基础。同时本项目的实施也将再矿化从防龋手段的研究发展为龋损修复方法的研究

Dental caries is the most prevalent oral disease in the world, posing a serious threat to oral and general health. The repairment of caries lesions is one of the most common and important dental clinical treatment. However, there are still some problems need to be solved in the current treatment such as filling microleakage, excitant to pulp, material aging and so on. According to biomimetic mineralization rationale, we put forward the hypothesis that biomimetic mineralization can cause the regeneration of tooth hard tissue in carious lesions in situ, which may become a new ideal repairment mode of dental carious lesions. However, so far there is no evidences to prove this hypothesis, the application research of biomimetic mineralization in this fields havn't been carried out. It is well known that amelogenin and dentin phosphoprotein act as a significant accelerant in biomineralized process of the tooth. Our previous experiments had obtained some functional peptides based amelogenin and dentin phosphoprotein, and for the first time it had been proved these functional peptides based on amelogenin and dentin phosphoprotein can enhance the remineralization of initial enamel carious lesion. In view of successful synthesis of hydroxyapatite induced by the polymer in vitro, by molecular biotechnology the current project will modify previous functional peptides based on amelogenin and dentin phosphoprotein to become a kind of biomimetic mineralization peptides, and test the potential of modified peptides in repairing the enamel / dentin carious lesion by biomimetic regeneration teeth hard tissue. Parameters of Biomimetic mineralization peptides delivery will be systematically optimized, including the form of calcium and phosphate used, and the target interface between Biomimetic mineralization peptidesand dental caries. Finally, the efficiency of repairment to carious lesions in situ is evaluated and its mechanism is explored. This study will provide the theoretical basis for the application of biomimetic mineralization peptides to repair the enamel/dentin carious lesion via biomimetic regeneration of teeth hard tissue. And the study will lay the solid foundation for breaking new ground in caries repairment and treatment. At the meantime, the implementation of this project will push the remineralization as a anticaries method into a repairment method to carious lesion through biomimetic process.