龋病修复是口腔临床最常见的工作，目前存在边缘微渗漏、牙髓刺激、粘接不良等问题。仿生矿化已展现出解决上述难题的潜能，但包含仿生矿化要素的仿生矿化微体系研发仍是全球面临的挑战。课题组前期开展牙体硬组织仿生矿化研究，已获得具有自主知识产权的仿生矿化功能多肽，文献报道及预实验提示，该多肽具有促牙本质再生潜能。本项目创新性提出基于仿生功能多肽促牙本质仿生矿化和修复性再生的潜能，借助生物活性玻璃的载药性、界面结合性及钙磷储备性，构建仿生功能多肽/生物活性玻璃复合体。通过检测复合体基本性能、促仿生矿化能力及对人牙髓细胞行为的影响，筛选稳定、安全、具有“仿生矿化-修复性牙本质再生”双重效应的复合体，探究其促仿生矿化的分子机制及调控细胞行为的信号通路，动物模型综合评价其原位修复牙本质效果。本项目提供了更易于临床转化的牙本质龋损修复新思路，拓宽了多肽的仿生研究领域，切实提高了功能多肽的牙本质龋损原位修复效果。

The common dentin caries treatments utilizing prosthodontic materials have the problems of the microleakage, pulp stimulation and poor adhesion. Biomimetic recovery approach has shown the potential to solve these problems in dentin caries treatment. However, it is still a global challenge to develop a mature micro-system involving key components including nuclearation stabilizer and endogenous calcium/phosphate. Researches on biomimetic mineralization of tooth hard tissue have been carried out previously by our group, and the biomimetic functional peptides with independent intellectual property have been obtained. Literature reports and preliminary experiments both supported these peptides own the potential to promote dentin regeneration. This project innovatively utilizing these peptids’ potential of biomimetic mineralization and reparative regeneration of dentin, combined with the bioactive glass (BGs) acting as drug loads, interface bonds and calcium/ phosphorus cargos, to obtain biomimetic functional peptide/ BGs complex. All candidates will be firstly screened by stability, biocompatibility and drug loading capability assays. Three different in vitro model, single demineralized collagen, three- dimensional demineralized collagen and caries-like dentin, will be used gradually to study the biomimetic mineralization ability of candidate complexes. The efficacy on proliferation and differentiation of human dental pulp cells will be studied in co-culture system and be verified by subcutaneous implantation. After all these assays, the idealist complex with dual-effects of "biomimetic mineralization-reparative regeneration" will selected, the in vivo dual-effects in the carious cavity will be comprehensively evaluated in animal experiments. In the same time, molecular dynamic simulation and high throughput transcriptome techniques will be used in this project, combined with traditional microscopic observations, crystal characterizations and techniques of cellular molecular biology, to explore the molecular mechanisms of the dual-effects of this novel biomimetic functional peptide/ BGs complex. The project provides a new approach to treat dentin caries which is also easier to be clinical transformed. In addition, this project expands the research filed of these peptides from remineralization studies to bioremediation studies involving cell biological behavior regulation. Last but not least, this project can effectively improve the in situ recovery efficacy of the biomimetic functional peptides on dentin caries, which is likely to lay a foundation for futher path of dentin repair. Thus, in conclusion, this project has important practical significance for the research of the dentin caries treatment.