新型隐球酵母细胞表面的葡萄糖受体和渗透压感应蛋白（sensors）尚未被描述。本小组的前期研究发现隐球菌位于细胞膜上的四跨膜蛋白（tetraspanin）Tsp2-1参与了葡萄糖感应和抗高渗透压、高温和氧化等胁迫。本计划拟采用"膜蛋白酵母双杂交(MYTH)"或者Co-IP等技术手段，获得在此等条件下与Tsp2-1相互作用的蛋白组（interactome）信息，进而鉴定出隐球菌对高浓度葡萄糖和高渗高温等胁迫条件的感应受体和信号产生的早期步骤；探讨Tsp2-1与已知的经典信号传导通路的关系；回答隐球菌中是否存在应答H2O2、SDS和高温等胁迫的信号通路等问题。由于Tsp2-1是膜蛋白，本计划将有助于揭示隐球菌与环境/宿主识别、相互作用的最初过程，为阐明隐球菌致病性机制提供新数据。

Sensors for glucose and other stress conditions on the cell surface in the pathogenic yeast Cryptococcus neoformans have not been described.In a previous study, we found that a membrane protein belonging to the tetraspanin superfamily, named Tsp2-1 in C. neoformans, was involved in glucose sensing and the reposne to osmotic pressure, high temperature and oxidative stress. Utilizing the modified membrane yeast two-hybrid technique (MYTH),this proposal is intended to mapping the functional Tsp2-1 interactome first. Based on the interactome, we are going to identify the putative glucose sensor, and proteins responsible for the signals against osmatic,heat, SDS and oxidative presures.And the interactome map will also indicate the corelationship of Tsp2-1 to the classic signal transduction pathways such as G-protein-cAMP-PKA and HOG MAPK pathways. And we are also interested in testing the hypothesis that there might be undescribed signal pathways for heat,osmatic,SDS and oxidative stress conditions in C. neoformans. This work will shed new light on the pathogenic mechanism in the fungus.