三七素（β-ODAP）是一种非蛋白氨基酸，是三七重要的止血功能成分。研究三七素的生物合成有助于加深我们对三七品质形成机理的理解。BIA是β-ODAP生物合成的直接前体，而BIA则是由丝氨酸O-乙酰转移酶（SAT）和BIA合酶 （BIAS）催化L-丝氨酸而形成的；然后由一种未知酶的催化BIA合成DAPA（2,3-二氨基丙酸），最后由DAPA N-草酰转移酶催化DAPA和草酰-CoA而形成β-ODAP；而草酰-CoA则是由草酰-CoA合成酶（OCS）催化草酸而来。针对三七素生物合成途径不清楚、关键酶基因未知、积累规律和环境因素影响缺乏了解等科学问题，本项目拟通过关键酶（OCS，SAT，BIAS）基因克隆和功能验证、HLCP检测三七素含量变化和基因表达关系、干旱胁迫对三七素积累关系等内容的研究，将探明三七素积累和分布规律，并部分解析其生物合成途径，深入了解三七品质形成机理。

Dencinchine (β-ODAP) is an important active component with emostatic function in medical plant Panax notoginseng. Studying on the dencinchine biosynthesis will help us understanding the quality of P. notoginseng. Previous studys had shown that, BIA（β-(isoxazolin-5-on-2-yl)-L-alanine is the direct precursor of β-ODAP and comes from L-serine catalyzed by serine O-acetyltransferase (SAT) and BIA synthase (BIAS); then BIA cztalyed by another unkonwn enzyme and 2,3-diaminopropionate N-oxalyltransferase for the formation of β-ODAP. The last step reaction alse need oxalyl-CoA, which comes from acetate and catalyzed by oxalyl-CoA synthetase. Until now, the pathway of dencinchine biosynthesis and the involved enzmes and genes are still absolutely unkown. For elucidate the biosynthesis pathway of dencinchine in P. notoginseng, three keys enzyme (OCS, SAT and BIAS) genes will be cloned, their catalytic characteristics of the enzymes and the functions of their genes will be detected by heterologous expression in yeast expression system and knockouted using Agrobacterium rhizogenes-mediated genetic transformation, respectively. Furthermore, the temporal and spatial expression of genes and the effects of environment factors on their expression will be detected by Real-time PCR. The results of this study will provide a full understanding of biosynthesis of dencinchine and helpful information on the quality of P. notoginseng.