灯盏花[Erigeron breviscapus (Vant.) Hand.-Mazz.]是最具云南地方特色药用植物之一，能够特异性地积累高含量的灯盏乙素(6-OH黄酮的葡糖苷酸) ，其生物合成最后两步反应机理尚未完全明确。基于基因组和转录组测序，项目组已经获得了催化灯盏乙素生物合成最后两步关键反应酶（F6H和F7GAT）的候选基因序列，并将很快鉴定出其功能。本项目拟将灯盏乙素途径的所有基因整合入酿酒酵母中，在酵母工程菌中重构完整的灯盏乙素合成途径并优化酵母工程菌，建立灯盏乙素工业发酵平台，实现灯盏花由传统种植业向现代发酵工业跨越。

Erigeron breviscapus (Vant.) Hand.-Mazz. is one of medicinal plants with regional feature of Yunnan Province, which particularly and highly accumulates scutellarin, a kind of 6-OH flavone glucuronide derivatives, the biosynthesis mechanism of scutellarin is still unclear. Our group had found the candidate genes which might encode flavone 6-hydroxylase (F6H) and flavonoid 7-O- glucuronosyltransferase (F7GAT), respectively, and involved in the last two key reactions, hydrolation at 6-C and glucuronidation at 7-C of scutellarin. In this study, we will transform all the genes of the whole biosynthetic pathway of scutellarin into engineered yeast and optimizing the system to lay the foundation of industrial production of scutellarin. This project will contribute to the transition from traditional plants to high-efficiency fermenting industry.