肝癌是严重威胁人类健康的恶性肿瘤之一，发病机制是受多因素影响、多基因调控、多信号通路参与的复杂网络体系，其根源是基因表达异常。前期研究克隆、筛选到一个新的肝癌相关基因HTA。初步研究发现沉默HTA基因后，HepG2细胞增殖受抑制、倍增时间延长、体内成瘤性降低等。随后采用RACE扩增、Northern blot验证获得该基因的全长cDNA序列。本研究拟大样本检测不同组织、外周血标本，结合临床资料进一步分析、确定HTA与肝癌发病进程、病理分期、临床预后相关性，评估HTA作为肝癌标志物的临床应用价值；构建HTA过表达、沉默表达细胞系，经体内、体外实验，从正、反两方面探讨HTA的生物学功能；经与全基因组芯片杂交、经生物信息学分析等方法找出HTA切入的信号通路和交互作用的关键分子。通过对HTA的系统研究，试图从一个全新的视野为阐明肝癌的发病机制提供理论依据，为肝癌的诊断、治疗提供新的靶点。

Hepatocellular carcinoma is one of the serious malignant tumors and threaten to human beings. The pathogenesis of hepatocellular carcinoma is a complex network system involved in many factors,genes regulation and signal pathways, the root is abnormal gene expression. Previous study have cloned and screened a hepatoma associated gene, HTA. Preliminary research suggested that silence HTA gene in HepG2 can inhibit proliferation, prolong doubling time, promote apoptosis and reduce tumorigenicity. Then using RACE amplified HTA gene full-length cDNA sequence,and tested by Northern blot. The purpose of this study is to analyse the correlation of HTA expression with hepatocellular carcinoma through detection large scale clinical samples; To construct HTA over expression and silent expression cell lines, study HTA by in vivo and in vitro experiments, from positive and negative aspects to analyse the biological function of HTA; Find out the signal pathways and key molecules that interact with HTA by hybridization with genome microarray, bioinformatics and other methods.Based on the comprehensive research of HTA, try to provide a theoretical basis for elucidating the pathogenesis, and provide a new target for diagnosis and treatment of hepatocellular carcinoma.